LOCUS M13KO7 8668 bp ss-DNA circular 10-OCT-2015 DEFINITION M13KO7 Helper Phage. ACCESSION VERSION .2 KEYWORDS . COMMENT Sequenced by Antibody Design Labs. FEATURES Location/Qualifiers gene join(8267..8668,1..831) /gene="II" /locus_tag="M13p01" /db_xref="GeneID:927328" CDS join(8267..8668,1..831) /gene="II" /locus_tag="M13p01" /note="The pII protein binds to the intergenic region in double-stranded, supercoiled DNA (replicative form) and nicks the positive strand. The resulting free 3' OH terminus serves as a primer for new strand synthesis using host factors (Rep helicase, DNA polymerase III). After a full round of replication, pII circularizes the displaced DNA strand. Newly synthesized (+) single stranded DNA is either converted to RF DNA or packaged as genomes, depending on the concentrations of ssDNA binding protein pV and a separately expressed fragment of pII termed pX. Functions: DNA replication initiation" /codon_start=1 /transl_table=11 /product="replication initiation protein" /protein_id="NP_510885.1" /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV DFSSQRPDWYNEPVLKIA*" /db_xref="GI:17426218" /db_xref="GeneID:927328" gene 496..831 /gene="X" /locus_tag="M13p02" /db_xref="GeneID:927329" CDS 496..831 /gene="X" /locus_tag="M13p02" /note="Protein pX is synthesized from an in-frame internal initiation codon in gene II and is identical to the C-terminal third of pII. Required for the accumulation of (+) single stranded genomic DNA." /codon_start=1 /transl_table=11 /product="gene X product" /protein_id="NP_510886.1" /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW YNEPVLKIA" /db_xref="GI:17426219" /db_xref="GeneID:927329" gene 843..1106 /gene="V" /locus_tag="M13p03" /db_xref="GeneID:927330" CDS 843..1106 /gene="V" /locus_tag="M13p03" /codon_start=1 /transl_table=11 /product="helix destabilising protein" /protein_id="NP_510887.1" /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK" /db_xref="GI:17426220" /db_xref="GeneID:927330" old_sequence 898 /gene="V" /locus_tag="M13p03" /citation=[1] /replace="g" gene 1108..1209 /gene="VII" /locus_tag="M13p04" /db_xref="GeneID:927331" CDS 1108..1209 /gene="VII" /locus_tag="M13p04" /note="pVII minor coat proteins of E. coli filamentous phages are located at the tip of the virion that first emerges from the host cell." /codon_start=1 /transl_table=11 /product="minor coat protein" /protein_id="NP_510888.1" /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR" /db_xref="GI:17426221" /db_xref="GeneID:927331" gene 1206..1304 /gene="IX" /locus_tag="M13p05" /db_xref="GeneID:927332" CDS 1206..1304 /gene="IX" /locus_tag="M13p05" /note="pVII minor coat proteins of E. coli filamentous phages are located at the tip of the virion that first emerges from the host cell." /codon_start=1 /transl_table=11 /product="minor coat protein" /protein_id="NP_510889.1" /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS" /db_xref="GI:17426222" /db_xref="GeneID:927332" gene 1301..1522 /gene="VIII" /locus_tag="M13p06" /db_xref="GeneID:927333" CDS 1301..1522 /gene="VIII" /locus_tag="M13p06" /note="Several thousand copies of the major coat protein form the tube containing the ssDNA genome to form the elongated body of filamentous phages." /codon_start=1 /transl_table=11 /product="major coat protein" /protein_id="NP_510890.1" /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY IGYAWAMVVVIVGATIGIKLFKKFTSKAS*" /db_xref="GI:17426223" /db_xref="GeneID:927333" gene 1579..2853 /gene="III" /locus_tag="M13p07" /db_xref="GeneID:927334" CDS 1579..2853 /gene="III" /locus_tag="M13p07" /note="Minor coat protein pIII is located at the tail of the filamentous phage particle. It is involved in termination of assembly and infectivity. In the absence of pIII abnormally long phage particles are formed and remain attached to host cells. In addition, pIII is involved in initiating infection of host cells by binding to pili. In the case of the Ff phages, pIII binds to the tip of F-pili and then interacts with the TolA protein. The assembly termination function maps to the C-terminus of pIII, whereas the receptor binding maps to the N-terminus. Ike and f1 proteins cannot replace one another. Functions: receptor binding, morphogenesis, structural." /codon_start=1 /transl_table=11 /product="attachment protein" /protein_id="NP_510891.1" /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKT LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFV CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFSAGKPYEFSIDCDKI NLFRGVFAFLLYVATFMYVFSTFANILRNKES" /db_xref="GI:17426224" /db_xref="GeneID:927334" gene 2856..3194 /gene="VI" /locus_tag="M13p08" /db_xref="GeneID:927335" CDS 2856..3194 /gene="VI" /locus_tag="M13p08" /note="The pVI proteins of Ff phages are minor coat proteins that are located at the end of the virion. They are involved in termination of assembly. In the absence of pVI, phage particles remain associated with the host membrane." /codon_start=1 /transl_table=11 /product="minor coat protein" /protein_id="NP_510892.1" /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ KIVSYLDWDK" /db_xref="GI:17426225" /db_xref="GeneID:927335" gene 3196..4242 /gene="I" /locus_tag="M13p09" /db_xref="GeneID:927336" CDS 3196..4242 /gene="I" /locus_tag="M13p09" /note="The pI protein of filamentous phages is involved in morphogenesis and may hydrolyze ATP. It is an inner membrane protein and interacts with the outer membrane protein pIV. The N-termini of members of this cluster share homology to the zonular occludens toxin (Zot) encoded by phage CTXphi. However, the toxin activity of Zot was mapped to the C terminus, which is divergent in the proteins in this cluster. pXI is the product of translational reinitiationin gene I and interacts with pI." /codon_start=1 /transl_table=11 /product="assembly protein" /protein_id="NP_510893.1" /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV SIKKGNSNEIVKCN" /db_xref="GI:17426226" /db_xref="GeneID:927336" gene 4220..5500 /gene="IV" /locus_tag="M13p10" /db_xref="GeneID:927337" CDS 4220..5500 /gene="IV" /locus_tag="M13p10" /note="pIV proteins of filamentous phages are multimeric proteins that form a channel in the outer membrane through which the phage particles exit the host cell. Function: morphogenesis." /codon_start=1 /transl_table=11 /product="phage assembly protein" /protein_id="NP_510894.1" /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNNQDYI DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL" /db_xref="GI:17426227" /db_xref="GeneID:927337" rep_origin 5488..5830 /note="M13 origin of replication (- +) (fragment)" rep_origin complement(6046..6591) /note="p15A origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(7140..7955) /gene="aph(3')-Ia" CDS complement(7140..7955) /gene="aph(3')-Ia" /note="kanR (confers resistance to kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" rep_origin 8092..8129 /note="M13 origin of replication (- +) (fragment)" misc_binding 5825..5830 /db_xref="REBASE:AvaI" misc_binding 2220..2225 /db_xref="REBASE:BamHI" misc_binding 7401..7406 /db_xref="REBASE:HindIII" ORIGIN 1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagccct 1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc 1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca 1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt 1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca 1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact 2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 2221 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 2701 tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta 2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg 2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt 2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc 2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg 3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact 3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc 3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg 3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg 3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat 3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt 3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat 3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat 3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt 3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg 3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct 3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat 3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat 3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat 3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta 3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt 3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg 4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct 4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat 4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc 4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt 4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt 4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg 4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc 4441 tgttttacgt gcaaataatt ttgatatggt aggttctaac ccttccatta ttcagaagta 4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga 4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac 4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa 4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt 4741 tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc 4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga 4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg 4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt 4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg 5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat 5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg 5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt 5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat 5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact 5341 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa 5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt 5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 5521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5821 ctatctcggg acggatcgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc 5881 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg 5941 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca 6001 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg 6061 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg 6121 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc 6181 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga 6241 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc 6301 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg 6361 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca 6421 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag 6481 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg 6541 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat 6601 cttattaagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc 6961 ggggatcgac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc 7021 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg 7081 ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat 7141 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata 7201 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat 7261 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct 7321 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact 7381 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag 7441 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc 7501 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa 7561 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat 7621 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca 7681 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt 7741 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac 7801 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca 7861 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc 7921 ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg 7981 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag 8041 agattttgag acacaacgtg gctttccccc cccccccctg caggtctcgg gctattcttt 8101 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 8161 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac 8221 aatcttcctg tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct 8281 agttttacga ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct 8341 gatagccttt gtagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag 8401 aacggttgaa tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga 8461 atctttacct acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt 8521 ttatccttgc gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt 8581 tggtacaacc gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt 8641 gccttgcctg tatgatttat tggatgtt //