LOCUS       X7-FC-hG1               5519 bp    DNA     circular
DEFINITION  Human IgG1 Fc TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,2827..3415,3431..3529)
            FT /number="17"
            FT color
            FT join(1100..1103,5279..5307)
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT 2444..2818
            FT /number="20"
            FT color
            FT join(1041..1099,1128..1814)
            FT /number="14"
            FT color
            FT 3603..4222
            FT /number="2"
            FT color
            FT join(4377..4517,4519..5237)
            FT /number="10"
            FT color
            FT join(1837..1847,1850..2349,2351,2354..2410)
            FT /number="7"
            FT translated
            FT join(1041..1103,1128..1812)
            FT /frame="3"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
                     /note="Transcription start (source PCEP4)"
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
                     /color="8"
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..1808
                     /codon_start=1
                     /translation="DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVV
                     DVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCK
                     VSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVE
                     WESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQ
                     KSLSLSPGK"
                     /gene
                     /label=hG1-FC
                     /note
     misc_feature    1809..1814
                     /gene
                     /label=Stop
                     /note
     misc_binding    1815..1820
                     /db_xref="REBASE:NheI"
     primer_bind     complement(1883..1902)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2347..2353
                     /label=A7
                     /note="see Bochkov 2006"
     misc_binding    2432..2437
                     /db_xref="REBASE:XbaI"
     misc_binding    2438..2443
                     /db_xref="REBASE:SalI"
     misc_binding    2819..2826
                     /db_xref="REBASE:NotI"
     polyA_signal    3431..3529
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3546..3551
                     /db_xref="REBASE:BglII"
     misc_binding    3552..3557
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3603..4222)
                     /label=pMB1
                     /gene="pBR322 origin"
     CDS             complement(4377..5237)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5279..5307)
                     /label=AmpR_Promoter
     misc_binding    5370..5375
                     /db_xref="REBASE:BglII"
     sig_peptide     1041..1103
                     /gene
                     /label=pelBK
                     /note
     misc_feature    1837..2410
                     /gene
                     /label=IRES
                     /note
     CDS             2444..2818
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene
                     /label=Zeo
                     /note
     misc_feature    2827..3415
                     /gene
                     /label=WPRE
                     /note
     misc_difference 4060
                     /gene
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagac aaaactcaca
     1141 catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc
     1201 caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg
     1261 acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc
     1321 ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg
     1381 tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtgtcca
     1441 acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg cagccccgag
     1501 aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac caggtcagcc
     1561 tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg gagagcaatg
     1621 ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct
     1681 tcctctatag caagctcacc gtggacaaga gcaggtggca gcaggggaac gtcttctcat
     1741 gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc tccctgtctc
     1801 cgggtaaata aacagctagc ggttccaacc ttggttcccc tctccctttc ccccccctaa
     1861 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     1921 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     1981 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2041 gaaggaagca gttcctctgg gagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2101 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     2161 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     2221 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     2281 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     2341 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2401 acgatgataa gcttgccaca acccgggatc ctctagagtc gacatggcca agttgaccag
     2461 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     2521 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     2581 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     2641 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     2701 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     2761 cgccctgcgc gacccggccg gcaactgcgt gcactttgtg gcagaggagc aggactgagc
     2821 ggccgcaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3001 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3061 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3121 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3181 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     3241 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3301 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3361 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcagatc
     3421 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3481 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     3541 ctcggagatc taagcttcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4381 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4441 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4501 gctgcaatga taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag
     4561 ccagccggaa gcgccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4621 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4681 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4741 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4801 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4861 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4921 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4981 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5041 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5101 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5161 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5221 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5341 cgcacatttc cccgaaaagt gccacctgaa gatctataca ttgaatcaat attggcaatt
     5401 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     5461 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccat
//
