LOCUS       TGEX-FC                 4019 bp    DNA     circular
DEFINITION  Antibody expression vector TGEX-FC, complete sequence
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     Antibody expression vector TGEX-FC, complete sequence
            FT color
            FT join(5..585,607..608,609..611,1931..2029)
            FT /number="17"
            FT color
            FT 2103..2722
            FT /number="2"
            FT color
            FT join(2877..3017,3019..3737)
            FT /number="10"
            FT color
            FT 3779..3807
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1051..1096,1209..1254,1182..1191,1255..1584,1192,
            FT 1585..1907)
            FT /number="14"
            FT color
            FT 1025..1050
            FT /number="20"
            FT color
            FT join(5..585,607..608,609..611,1931..2029)
            FT /number="17"
            FT color
            FT 2103..2722
            FT /number="2"
            FT color
            FT join(2877..3017,3019..3737)
            FT /number="10"
            FT color
            FT 3779..3807
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1051..1096,1209..1254,1182..1191,1255..1584,1192,
            FT 1585..1907)
            FT /number="14"
            FT color
            FT 1025..1050
            FT /number="20"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_promoter
     misc_feature    603
                     /label=Start
                     /note="Transcription start"
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_feature    1025..1192
                     /label=IGHV1-2
                     /note="Partial human mRNA encoding leader sequence"
     CDS             1051..1096
                     /codon_start=1
                     /translation="MDWTWRILFLVAAAT"
     misc_feature    1097..1098
                     /label=SD
     misc_feature    1180..1181
                     /label=SA
     misc_binding    1182..1187
                     /db_xref="REBASE:BssHII"
     CDS             1184..1192
                     /codon_start=1
                     /translation="AHS"
     misc_feature    1193
                     /label=VH_Start
     misc_binding    1202..1207
                     /db_xref="REBASE:BsmBI"
     CDS             1209..1907
                     /codon_start=1
                     /translation="EPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV
                     TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS
                     DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH
                     NHYTQKSLSLSPGK*"
                     /label=IgG1-Fc
     misc_binding    1908..1915
                     /db_xref="REBASE:NotI"
     polyA_signal    1931..2029
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(1965..1988)
                     /label=tgex-R2
     misc_binding    2046..2051
                     /db_xref="REBASE:BglII"
     misc_binding    2052..2057
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(2103..2722)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2559)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(2877..3737)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(3779..3807)
                     /label=AmpR_Promoter
     misc_binding    3870..3875
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcatcccc tgagagctcc gttcctcacc atggactgga cctggagaat cctcttcttg
     1081 gtggcagcag ccacaggtaa gaggctccct agtcccagtg atgagaaaga gattgagtcc
     1141 agtccaggga gatctcatcc acttctgtgt tctctccaca ggcgcgcact cccaggtgca
     1201 ccgtctctga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg
     1261 aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga
     1321 tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg
     1381 tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg
     1441 aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact
     1501 ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg
     1561 agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc
     1621 catcccggga tgagctgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct
     1681 atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga
     1741 ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctatagcaag ctcaccgtgg
     1801 acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat gaggctctgc
     1861 acaaccacta cacgcagaag agcctctccc tgtctccggg taaatgagcg gccgcagatc
     1921 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     1981 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     2041 ctcggagatc taagcttcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3001 gctgcaatga taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag
     3061 ccagccggaa gcgccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3181 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3481 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3781 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3841 cgcacatttc cccgaaaagt gccacctgaa gatctataca ttgaatcaat attggcaatt
     3901 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     3961 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccat
//

