LOCUS       TGEX-FH-hG1             5183 bp    DNA     circular
DEFINITION  Human IgG1 CH1 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,2491..3079,3095..3193)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,4943..4971)
            FT /number="1"
            FT color
            FT 2108..2482
            FT /number="20"
            FT color
            FT join(1041..1099,1132..1478)
            FT /number="14"
            FT translated
            FT 2108..2482
            FT /frame="2"
            FT color
            FT 3267..3886
            FT /number="2"
            FT color
            FT join(1501..1511,1514..2013,2015,2018..2074)
            FT /number="7"
            FT color
            FT join(4041..4181,4183..4901)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..1475
                     /codon_start=1
                     /translation="ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSG
                     ALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS
                     CGGGSPHHHHHHG*"
                     /label=huG1-CH1-HIS-Tag
                     /note="truncated"
     misc_binding    1443..1448
                     /db_xref="REBASE:BamHI"
     misc_binding    1452..1469
                     /gene
                     /label=HIS-Tag
                     /note
     misc_feature    1473..1478
                     /gene
                     /label=Stop
                     /note
     misc_binding    1479..1484
                     /db_xref="REBASE:NheI"
     misc_feature    1501..2074
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1547..1566)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2011..2017
                     /label=A7
                     /note
     misc_binding    2096..2101
                     /db_xref="REBASE:XbaI"
     misc_binding    2102..2107
                     /db_xref="REBASE:SalI"
     CDS             2108..2482
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    2483..2490
                     /db_xref="REBASE:NotI"
     misc_feature    2491..3079
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3095..3193
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3210..3215
                     /db_xref="REBASE:BglII"
     misc_binding    3216..3221
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3267..3886)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(3723)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4041..4901)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(4943..4971)
                     /label=AmpR_Promoter
     misc_binding    5034..5039
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagca tccaccaagg
     1141 gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc acagcggccc
     1201 tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg
     1261 ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga ctctactccc
     1321 tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac atctgcaacg
     1381 tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tgagcccaaa tcttgtggtg
     1441 gcggatcccc tcaccatcac catcatcatg gctaaacagc tagcggttcc aaccttggtt
     1501 cccctctccc tttccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1561 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1621 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1681 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctgggagctt cttgaagaca
     1741 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1801 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1861 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     1921 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     1981 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     2041 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg gatcctctag
     2101 agtcgacatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2161 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2221 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2281 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     2341 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     2401 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     2461 tgtggcagag gagcaggact gagcggccgc aatcaacctc tggattacaa aatttgtgaa
     2521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2881 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3061 ctttgggccg cctccccgca gatcccccga cctcgacctc tggctaataa aggaaattta
     3121 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3181 gggcaaatca tttggtcgag atccctcgga gatctaagct tcatgtgagc aaaaggccag
     3241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4141 gagggcttac catctggccc cagtgctgca atgataccgc gtgacccacg ctcaccggct
     4201 ccagatttat cagcaataaa ccagccagcc ggaagcgccg agcgcagaag tggtcctgca
     4261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaagatcta
     5041 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5101 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5161 ctcatgtcca atatgaccgc cat
//
