LOCUS       TGEX-FH                 3668 bp    DNA     circular
DEFINITION  Antibody expression vector TGEX-FH, complete sequence
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     Antibody expression vector TGEX-FH, complete sequence
            FT color
            FT join(5..585,607..608,609..611,1580..1678)
            FT /number="17"
            FT color
            FT 1752..2371
            FT /number="2"
            FT color
            FT join(2526..2666,2668..3386)
            FT /number="10"
            FT color
            FT 3428..3456
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1051..1096,1182..1191,1192,1209..1532,1551..1556)
            FT /number="14"
            FT color
            FT 1025..1050
            FT /number="20"
            FT color
            FT 1533..1550
            FT /number="26"
            FT color
            FT join(5..585,607..608,609..611,1580..1678)
            FT /number="17"
            FT color
            FT 1752..2371
            FT /number="2"
            FT color
            FT join(2526..2666,2668..3386)
            FT /number="10"
            FT color
            FT 3428..3456
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1051..1096,1182..1191,1192,1209..1532,1551..1556)
            FT /number="14"
            FT color
            FT 1025..1050
            FT /number="20"
            FT color
            FT 1533..1550
            FT /number="26"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_promoter
     misc_feature    603
                     /label=Start
                     /note="Transcription start"
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_feature    1025..1192
                     /label=IGHV1-2
                     /note="Partial human mRNA encoding leader sequence"
     CDS             1051..1096
                     /codon_start=1
                     /translation="MDWTWRILFLVAAAT"
     misc_feature    1097..1098
                     /label=SD
     misc_feature    1180..1181
                     /label=SA
     misc_binding    1182..1187
                     /db_xref="REBASE:BssHII"
     CDS             1184..1192
                     /codon_start=1
                     /translation="AHS"
     misc_feature    1193
                     /label=VH_Start
     misc_binding    1202..1207
                     /db_xref="REBASE:BsmBI"
     CDS             1209..1211
                     /codon_start=1
                     /translation="A"
     CDS             1212..1556
                     /codon_start=1
                     /translation="STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC
                     GGGSPHHHHHHG*"
                     /label=huG1-CH1
                     /note="with C-terminal HIS tag"
     misc_binding    1524..1529
                     /db_xref="REBASE:BamHI"
     misc_feature    1533..1550
                     /label=HIS_tag
     misc_binding    1557..1564
                     /db_xref="REBASE:NotI"
     polyA_signal    1580..1678
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(1614..1637)
                     /label=tgex-R2
     misc_binding    1695..1700
                     /db_xref="REBASE:BglII"
     misc_binding    1701..1706
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(1752..2371)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2208)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(2526..3386)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(3428..3456)
                     /label=AmpR_Promoter
     misc_binding    3519..3524
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcatcccc tgagagctcc gttcctcacc atggactgga cctggagaat cctcttcttg
     1081 gtggcagcag ccacaggtaa gaggctccct agtcccagtg atgagaaaga gattgagtcc
     1141 agtccaggga gatctcatcc acttctgtgt tctctccaca ggcgcgcact cccaggtgca
     1201 ccgtctctgc atccaccaag ggcccatcgg tcttccccct ggcaccctcc tccaagagca
     1261 cctctggggg cacagcggcc ctgggctgcc tggtcaagga ctacttcccc gaaccggtga
     1321 cggtgtcgtg gaactcaggc gccctgacca gcggcgtgca caccttcccg gctgtcctac
     1381 agtcctcagg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca
     1441 cccagaccta catctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagaaag
     1501 ttgagcccaa atcttgtggt ggcggatccc ctcaccatca ccatcatcat ggctgagcgg
     1561 ccgcagatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     1621 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     1681 tcgagatccc tcggagatct aagcttcatg tgagcaaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2641 ggccccagtg ctgcaatgat accgcgtgac ccacgctcac cggctccaga tttatcagca
     2701 ataaaccagc cagccggaag cgccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2821 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3121 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3481 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaag atctatacat tgaatcaata
     3541 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
     3601 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
     3661 accgccat
//

