LOCUS       TGEX-HC-dG1-Zeo         5831 bp    DNA     circular
DEFINITION  Dog IgG1 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3139..3727,3743..3841)
            FT /number="17"
            FT color
            FT join(1100..1103,5591..5619)
            FT /number="1"
            FT color
            FT join(612..1000,2149..2722)
            FT /number="8"
            FT color
            FT 2756..3130
            FT /number="20"
            FT color
            FT join(1041..1099,1128..2126)
            FT /number="14"
            FT color
            FT 3915..4534
            FT /number="2"
            FT color
            FT join(4689..4829,4831..5549)
            FT /number="10"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /note
                     /color="1"
     misc_feature    603
                     /label=Start
                     /note="Transcription start (source PCEP4)"
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2123
                     /codon_start=1
                     /translation="ASTTAPSVFPLAPSCGSTSGSTVALACLVSGYFPEPVTVSWNSG
                     SLTSGVHTFPSVLQSSGLHSLSSMVTVPSSRWPSETFTCNVVHPASNTKVDKPVFNEC
                     RCTDTPPCPVPEPLGGPSVLIFPPKPKDILRITRTPEVTCVVLDLGREDPEVQISWFV
                     DGKEVHTAKTQSREQQFNGTYRVVSVLPIEHQDWLTGKEFKCRVNHIDLPSPIERTIS
                     KARGRAHKPSVYVLPPSPKELSSSDTVSITCLIKDFYPPDIDVEWQSNGQQEPERKHR
                     MTPPQLDEDGSYFLYSKLSVDKSRWQQGDPFTCAVMHETLQNHYTDLSLSHSPGK*"
                     /label=dG1
     misc_feature    2121..2126
                     /gene
                     /label=Stop
                     /note
     misc_binding    2127..2132
                     /db_xref="REBASE:NheI"
     misc_feature    2149..2722
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2195..2214)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2659..2665
                     /label=A7
                     /note="see Bochkov 2006"
     misc_binding    2744..2749
                     /db_xref="REBASE:XbaI"
     misc_binding    2750..2755
                     /db_xref="REBASE:SalI"
     CDS             2756..3130
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3131..3138
                     /db_xref="REBASE:NotI"
     misc_feature    3139..3727
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3743..3841
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3858..3863
                     /db_xref="REBASE:BglII"
     misc_binding    3864..3869
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3915..4534)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4371)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4689..5549)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5591..5619)
                     /label=AmpR_Promoter
     misc_binding    5682..5687
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagcc tccaccacgg
     1141 ccccctcggt tttcccactg gcccccagct gcgggtccac ttccggctcc acggtggccc
     1201 tggcctgcct ggtgtcaggc tacttccccg agcctgtaac tgtgtcctgg aattccggct
     1261 ccttgaccag cggtgtgcac accttcccgt ccgtcctgca gtcctcaggg cttcactccc
     1321 tcagcagcat ggtgacagtg ccctccagca ggtggcccag cgagaccttc acctgcaacg
     1381 tggtccaccc agccagcaac actaaagtag acaagccagt gttcaatgaa tgcagatgca
     1441 ctgatacacc cccatgccca gtccctgaac ctctgggagg gccttcggtc ctcatctttc
     1501 ccccgaaacc caaggacatc ctcaggatta cccgaacacc cgaggtcacc tgtgtggtgt
     1561 tagatctggg ccgtgaggac cctgaggtgc agatcagctg gttcgtggat ggtaaggagg
     1621 tgcacacagc caagacccag tctcgtgagc agcagttcaa cggcacctac cgtgtggtca
     1681 gcgtcctccc cattgagcac caggactggc tcacagggaa ggagttcaag tgcagagtca
     1741 accacataga cctcccgtct cccatcgaga ggaccatctc taaggccaga gggagggccc
     1801 ataagcccag tgtgtatgtc ctgccgccat ccccaaagga gttgtcatcc agtgacacag
     1861 tcagcatcac ctgcctgata aaagacttct acccacctga cattgatgtg gagtggcaga
     1921 gcaatggaca gcaggagccc gagaggaagc accgcatgac cccgccccag ctggacgagg
     1981 acgggtccta cttcctgtac agcaagctct ctgtggacaa gagccgctgg cagcagggag
     2041 accccttcac atgtgcggtg atgcatgaaa ctctacagaa ccactacaca gatctatccc
     2101 tctcccattc tccgggtaaa taaacagcta gcggttccaa ccttggttcc cctctccctt
     2161 tcccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     2221 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     2281 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     2341 gttgaatgtc gtgaaggaag cagttcctct gggagcttct tgaagacaaa caacgtctgt
     2401 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     2461 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     2521 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     2581 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     2641 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     2701 cctttgaaaa acacgatgat aagcttgcca caacccggga tcctctagag tcgacatggc
     2761 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     2821 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     2881 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     2941 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     3001 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     3061 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcactttg tggcagagga
     3121 gcaggactga gcggccgcaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3541 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3721 tccccgcaga tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa
     3781 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     3841 tggtcgagat ccctcggaga tctaagcttc atgtgagcaa aaggccagca aaaggccagg
     3901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4801 tctggcccca gtgctgcaat gataccgcgt gacccacgct caccggctcc agatttatca
     4861 gcaataaacc agccagccgg aagcgccgag cgcagaagtg gtcctgcaac tttatccgcc
     4921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aagatctata cattgaatca
     5701 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     5761 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     5821 atgaccgcca t
//
