LOCUS       TGEX-HC-dG2-Zeo         5843 bp    DNA     circular
DEFINITION  Dog IgG2 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3151..3739,3755..3853)
            FT /number="17"
            FT color
            FT join(1100..1103,5603..5631)
            FT /number="1"
            FT color
            FT join(612..1000,2161..2734)
            FT /number="8"
            FT color
            FT 2768..3142
            FT /number="20"
            FT color
            FT join(1041..1099,2133..2138)
            FT /number="14"
            FT color
            FT 3927..4546
            FT /number="2"
            FT color
            FT join(4701..4841,4843..5561)
            FT /number="10"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /note
                     /color="1"
     misc_feature    603
                     /label=Start
                     /note="Transcription start (source PCEP4)"
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
                     /color="8"
     primer_bind     974..995
                     /label=tgex3_s
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2135
                     /codon_start=1
                     /translation="ASTTAPSVFPLAPSCGSTSGSTVALACLVSGYFPEPVTVSWNSG
                     SLTSGVHTFPSVLQSSGLYSLSSMVTVPSSRWPSETFTCNVAHPASKTKVDKPVPKRE
                     NGRVPRPPDCPKCPAPEMLGGPSVFIFPPKPKDTLLIARTPEVTCVVVDLDPEDPEVQ
                     ISWFVDGKQMQTAKTQPREEQFNGTYRVVSVLPIGHQDWLKGKQFTCKVNNKALPSPI
                     ERTISKARGQAHQPSVYVLPPSREELSKNTVSLTCLIKDFFPPDIDVEWQSNGQQEPE
                     SKYRTTPPQLDEDGSYFLYSKLSVDKSRWQRGDTFICAVMHEALHNHYTQESLSHSPG
                     K*"
                     /label=dG2
     misc_feature    2133..2138
                     /gene
                     /label=Stop
                     /note
     misc_binding    2139..2144
                     /db_xref="REBASE:NheI"
     misc_feature    2161..2734
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2207..2226)
                     /gene
                     /label=ires_rev
                     /note
     misc_feature    2671..2677
                     /label=A7
                     /note="see Bochkov 2006"
     misc_binding    2756..2761
                     /db_xref="REBASE:XbaI"
     misc_binding    2762..2767
                     /db_xref="REBASE:SalI"
     CDS             2768..3143
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3143..3150
                     /db_xref="REBASE:NotI"
     misc_feature    3151..3739
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3755..3853
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3870..3875
                     /db_xref="REBASE:BglII"
     misc_binding    3876..3881
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3927..4546)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4383)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4701..5561)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5603..5631)
                     /label=AmpR_Promoter
     misc_binding    5694..5699
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagcc tccaccacgg
     1141 ccccctcggt tttcccactg gcccccagct gcgggtccac ttccggctcc acggtggccc
     1201 tggcctgcct ggtgtcaggc tacttccccg agcctgtaac tgtgtcctgg aattccggct
     1261 ccttgaccag cggtgtgcac accttcccgt ccgtcctgca gtcctcaggg ctctactccc
     1321 tcagcagcat ggtgacagtg ccctccagca ggtggcccag cgagaccttc acctgcaacg
     1381 tggcccaccc ggccagcaaa actaaagtag acaagccagt gcccaaaaga gaaaatggaa
     1441 gagttcctcg cccacctgat tgtcccaaat gcccagcccc tgaaatgctg ggagggcctt
     1501 cggtcttcat ctttcccccg aaacccaagg acaccctctt gattgcccga acacctgagg
     1561 tcacatgtgt ggtggtggat ctggacccag aagaccctga ggtgcagatc agctggttcg
     1621 tggacggtaa gcagatgcaa acagccaaga ctcagcctcg tgaggagcag ttcaatggca
     1681 cctaccgtgt ggtcagtgtc ctccccattg ggcaccagga ctggctcaag gggaagcagt
     1741 tcacgtgcaa agtcaacaac aaagccctcc catccccgat cgagaggacc atctccaagg
     1801 ccagagggca ggcccatcag cccagtgtgt atgtcctgcc gccatcccgg gaggagttga
     1861 gcaagaacac agtcagcttg acatgcctga tcaaagactt cttcccacct gacattgatg
     1921 tggagtggca gagcaatgga cagcaggagc ctgagagcaa gtaccgcacg accccgcccc
     1981 agctggacga ggacgggtcc tacttcctgt acagcaagct ctctgtggac aagagccgct
     2041 ggcagcgggg agacaccttc atatgtgcgg tgatgcatga agctctacac aaccactaca
     2101 cacaggaatc cctctcccat tctccgggta aataaacagc tagcggttcc aaccttggtt
     2161 cccctctccc tttccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     2221 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     2281 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     2341 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctgggagctt cttgaagaca
     2401 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     2461 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     2521 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     2581 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     2641 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     2701 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg gatcctctag
     2761 agtcgacatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2821 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2881 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2941 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     3001 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3061 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3121 tgtggcagag gagcaggact gagcggccgc aatcaacctc tggattacaa aatttgtgaa
     3181 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3241 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3301 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3361 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3421 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3481 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3541 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3601 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3661 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3721 ctttgggccg cctccccgca gatcccccga cctcgacctc tggctaataa aggaaattta
     3781 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3841 gggcaaatca tttggtcgag atccctcgga gatctaagct tcatgtgagc aaaaggccag
     3901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4801 gagggcttac catctggccc cagtgctgca atgataccgc gtgacccacg ctcaccggct
     4861 ccagatttat cagcaataaa ccagccagcc ggaagcgccg agcgcagaag tggtcctgca
     4921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaagatcta
     5701 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5761 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5821 ctcatgtcca atatgaccgc cat
//
     5701 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5761 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5821 ctcatgtcca atatgaccgc cat
//
