LOCUS       TGEX-HC-dG3-Zeo         5837 bp    DNA     circular
DEFINITION  Dog IgG3 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3145..3733,3749..3847)
            FT /number="17"
            FT color
            FT join(1100..1103,5597..5625)
            FT /number="1"
            FT color
            FT join(612..1000,2155..2728)
            FT /number="8"
            FT color
            FT 2762..3136
            FT /number="20"
            FT color
            FT join(1041..1099,2127..2132)
            FT /number="14"
            FT color
            FT 3921..4540
            FT /number="2"
            FT color
            FT join(4695..4835,4837..5555)
            FT /number="10"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /note
                     /color="1"
     misc_feature    603
                     /label=Start
                     /note="Transcription start (source PCEP4)"
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex3-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2129
                     /codon_start=1
                     /translation="ASTTAPSVFPLAPSCGSQSGSTVALACLVSGYIPEPVTVSWNSV
                     SLTSGVHTFPSVLQSSGLYSLSSMVTVPSSRWPSETFTCNVAHPATNTKVDKPVAKEC
                     ECKCNCNNCPCPGCGLLGGPSVFIFPPKPKDILVTARTPTVTCVVVDLDPENPEVQIS
                     WFVDSKQVQTANTQPREEQSNGTYRVVSVLPIGHQDWLSGKQFKCKVNNKALPSPIEE
                     IISKTPGQAHQPNVYVLPPSRDEMSKNTVTLTCLVKDFFPPEIDVEWQSNGQQEPESK
                     YRMTPPQLDEDGSYFLYSKLSVDKSRWQRGDTFICAVMHEALHNHYTQISLSHSPGK*
                     "
                     /label=dG3
     misc_feature    2127..2132
                     /gene
                     /label=Stop
                     /note
     misc_binding    2133..2138
                     /db_xref="REBASE:NheI"
     misc_feature    2155..2728
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2201..2220)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2665..2671
                     /label=A7
                     /note="see Bochkov 2006"
     misc_binding    2750..2755
                     /db_xref="REBASE:XbaI"
     misc_binding    2756..2761
                     /db_xref="REBASE:SalI"
     CDS             2762..3137
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3137..3144
                     /db_xref="REBASE:NotI"
     misc_feature    3145..3733
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3749..3847
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3864..3869
                     /db_xref="REBASE:BglII"
     misc_binding    3870..3875
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3921..4540)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4377)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4695..5555)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5597..5625)
                     /label=AmpR_Promoter
     misc_binding    5688..5693
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagcc tccaccacgg
     1141 ccccctcggt tttcccactg gcccccagct gtgggtccca atccggctcc acggtggccc
     1201 tggcctgcct ggtgtcaggc tacatccccg agcctgtaac tgtgtcctgg aattccgtct
     1261 ccttgaccag cggtgtgcac accttcccgt ccgtcctgca gtcctcaggg ctctactccc
     1321 tcagcagcat ggtgacagtg ccctccagca ggtggcccag cgagaccttc acctgcaatg
     1381 tggcccaccc ggccaccaac actaaagtag acaagccagt ggccaaagaa tgcgagtgca
     1441 agtgtaactg taacaactgc ccatgcccag gttgtggcct gctgggaggg ccttcggtct
     1501 tcatctttcc cccaaaaccc aaggacatcc tcgtgactgc ccggacaccc acagtcactt
     1561 gtgtggtggt ggatctggac ccagaaaacc ctgaggtgca gatcagctgg ttcgtggata
     1621 gtaagcaggt gcaaacagcc aacacgcagc ctcgtgagga gcagtccaat ggcacctacc
     1681 gtgtggtcag tgtcctcccc attgggcacc aggactggct ttcagggaag cagttcaagt
     1741 gcaaagtcaa caacaaagcc ctcccatccc ccattgagga gatcatctcc aagaccccag
     1801 ggcaggccca tcagcctaat gtgtatgtcc tgccgccatc gcgggatgag atgagcaaga
     1861 atacggtcac cctgacctgt ctggtcaaag acttcttccc acctgagatt gatgtggagt
     1921 ggcagagcaa tggacagcag gagcctgaga gcaagtaccg catgaccccg ccccagctgg
     1981 atgaggatgg gtcctacttc ctatacagca agctctctgt ggacaagagc cgctggcagc
     2041 ggggagacac cttcatatgt gcggtgatgc atgaagctct acacaaccac tacacacaga
     2101 tatccctctc ccattctccg ggtaaataaa cagctagcgg ttccaacctt ggttcccctc
     2161 tccctttccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2221 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     2281 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     2341 aggtctgttg aatgtcgtga aggaagcagt tcctctggga gcttcttgaa gacaaacaac
     2401 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     2461 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     2521 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     2581 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     2641 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     2701 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccgggatcct ctagagtcga
     2761 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     2821 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     2881 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     2941 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     3001 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     3061 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc actttgtggc
     3121 agaggagcag gactgagcgg ccgcaatcaa cctctggatt acaaaatttg tgaaagattg
     3181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3301 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3361 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3421 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3541 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3601 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3661 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3721 gccgcctccc cgcagatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     3781 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3841 atcatttggt cgagatccct cggagatcta agcttcatgt gagcaaaagg ccagcaaaag
     3901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4801 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     4861 ttatcagcaa taaaccagcc agccggaagc gccgagcgca gaagtggtcc tgcaacttta
     4921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaga tctatacatt
     5701 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     5761 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     5821 tccaatatga ccgccat
//
