LOCUS       TGEX-HC-hG3-Zeo         5969 bp    DNA     circular
DEFINITION  Human IgG3 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3277..3865,3881..3979)
            FT /number="17"
            FT color
            FT join(1100..1103,5729..5757)
            FT /number="1"
            FT color
            FT 2894..3268
            FT /number="20"
            FT color
            FT join(1041..1099,1128..2264)
            FT /number="14"
            FT translated
            FT 2894..3268
            FT /frame="2"
            FT color
            FT 4053..4672
            FT /number="2"
            FT color
            FT join(2287..2297,2300..2799,2801,2804..2860)
            FT /number="7"
            FT color
            FT join(4827..4967,4969..5687)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2261
                     /codon_start=1
                     /translation="ASTKGPSVFPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSG
                     ALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKT
                     PLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCP
                     APELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNAK
                     TKPREEQYNSTFRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKTKGQPREP
                     QVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESSGQPENNYNTTPPMLDSDGSF
                     FLYSKLTVDKSRWQQGNIFSCSVMHEALHNRFTQKSLSLSPGK*"
                     /gene
                     /label=IgG3*01
                     /note
     misc_feature    2259..2264
                     /gene
                     /label=Stop
                     /note
     misc_binding    2265..2270
                     /db_xref="REBASE:NheI"
     misc_feature    2287..2860
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2333..2352)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2797..2803
                     /label=A7
                     /note
     misc_binding    2882..2887
                     /db_xref="REBASE:XbaI"
     misc_binding    2888..2893
                     /db_xref="REBASE:SalI"
     CDS             2894..3268
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3269..3276
                     /db_xref="REBASE:NotI"
     misc_feature    3277..3865
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3881..3979
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3996..4001
                     /db_xref="REBASE:BglII"
     misc_binding    4002..4007
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(4053..4672)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4509)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4827..5687)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5729..5757)
                     /label=AmpR_Promoter
     misc_binding    5820..5825
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagca tccaccaagg
     1141 gcccatcggt cttccccctg gcgccctgct ccaggagcac ctctgggggc acagcggccc
     1201 tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg
     1261 ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga ctctactccc
     1321 tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac acctgcaacg
     1381 tgaatcacaa gcccagcaac accaaggtgg acaagagagt tgagctcaaa accccacttg
     1441 gtgacacaac tcacacatgc ccacggtgcc cagagcccaa atcttgtgac acacctcccc
     1501 cgtgcccacg gtgcccagag cccaaatctt gtgacacacc tcccccatgc ccacggtgcc
     1561 cagagcccaa atcttgtgac acacctcccc cgtgcccaag gtgcccagca cctgaactcc
     1621 tgggaggacc gtcagtcttc ctcttccccc caaaacccaa ggataccctt atgatttccc
     1681 ggacccctga ggtcacgtgc gtggtggtgg acgtgagcca cgaagacccc gaggtccagt
     1741 tcaagtggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc
     1801 agtacaacag cacgttccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga
     1861 acggcaagga gtacaagtgc aaggtgtcca acaaagccct cccagccccc atcgagaaaa
     1921 ccatctccaa aaccaaagga cagccccgag aaccacaggt gtacaccctg cccccatccc
     1981 gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctacccca
     2041 gcgacatcgc cgtggagtgg gagagcagcg ggcagccgga gaacaactac aacaccacgc
     2101 ctcccatgct ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga
     2161 gcaggtggca gcaggggaac atcttctcat gctccgtgat gcatgaggct ctgcacaacc
     2221 gcttcacgca gaagagcctc tccctgtctc cgggtaaata aacagctagc ggttccaacc
     2281 ttggttcccc tctccctttc ccccccctaa cgttactggc cgaagccgct tggaataagg
     2341 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2401 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2461 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg gagcttcttg
     2521 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2581 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2641 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2701 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2761 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2821 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccgggatc
     2881 ctctagagtc gacatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     2941 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     3001 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     3061 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     3121 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     3181 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     3241 gcactttgtg gcagaggagc aggactgagc ggccgcaatc aacctctgga ttacaaaatt
     3301 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3361 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3421 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3481 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3541 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3601 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3661 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3721 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3781 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3841 atctcccttt gggccgcctc cccgcagatc ccccgacctc gacctctggc taataaagga
     3901 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     3961 atgggagggc aaatcatttg gtcgagatcc ctcggagatc taagcttcat gtgagcaaaa
     4021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgtga cccacgctca
     4981 ccggctccag atttatcagc aataaaccag ccagccggaa gcgccgagcg cagaagtggt
     5041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     5821 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     5881 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     5941 tattggctca tgtccaatat gaccgccat
//
