LOCUS       TGEX-HC-mG1-Zeo         5810 bp    DNA     circular
DEFINITION  Murine IgG1 (Mus musculus) TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3118..3706,3722..3820)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,5570..5598)
            FT /number="1"
            FT color
            FT 2735..3109
            FT /number="20"
            FT color
            FT join(1041..1099,1132..2105)
            FT /number="14"
            FT translated
            FT 2735..3109
            FT /frame="2"
            FT color
            FT 3894..4513
            FT /number="2"
            FT color
            FT join(2128..2138,2141..2640,2642,2645..2701)
            FT /number="7"
            FT color
            FT join(4668..4808,4810..5528)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2102
                     /codon_start=1
                     /translation="AKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSG
                     SLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC
                     GCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVE
                     VHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKG
                     RPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMD
                     TDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK*"
                     /gene
                     /label=mG1
                     /note="Mostly mG1*01"
     misc_difference 1299
                     /label=IGHG1-02
                     /note="Q"
     misc_difference 1347..1350
                     /label=IGHG1-02
                     /note="TW"
     misc_difference 1359..1361
                     /gene
                     /label=IGHG1*01
                     /note="E"
     misc_difference 1626..1628
                     /gene
                     /label=mG1*01
                     /note="Q"
     misc_difference 1644..1646
                     /gene
                     /label=mG1*01
                     /note="F"
     misc_difference 1869..1871
                     /gene
                     /label=mG1*01
                     /note
     misc_difference 1953..1955
                     /gene
                     /label=IGHG1-02
                     /note="N"
     misc_difference 1958..1960
                     /gene
                     /label=IGHG1-02
                     /note
     misc_feature    2100..2105
                     /gene
                     /label=Stop
                     /note
     misc_binding    2106..2111
                     /db_xref="REBASE:NheI"
     misc_feature    2128..2701
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2174..2193)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2638..2644
                     /label=A7
                     /note
     misc_binding    2723..2728
                     /db_xref="REBASE:XbaI"
     misc_binding    2729..2734
                     /db_xref="REBASE:SalI"
     CDS             2735..3109
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3110..3117
                     /db_xref="REBASE:NotI"
     misc_feature    3118..3706
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3722..3820
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3837..3842
                     /db_xref="REBASE:BglII"
     misc_binding    3843..3848
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3894..4513)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4350)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4668..5528)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5570..5598)
                     /label=AmpR_Promoter
     misc_binding    5661..5666
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagct aaaacgacac
     1141 ccccatctgt ctatccactg gcccctggat ctgctgccca aactaactcc atggtgaccc
     1201 tgggatgcct ggtcaagggc tatttccctg agccagtgac agtgacctgg aactctggat
     1261 ccctgtccag cggtgtgcac accttcccag ctgtcctgca gtctgacctc tacactctga
     1321 gcagctcagt gactgtcccc tccagcacct ggcccagcga aaccgtcacc tgcaacgttg
     1381 cccacccggc cagcagcacc aaggtggaca agaaaattgt gcccagggat tgtggttgta
     1441 agccttgcat atgtacagtc ccagaagtat catctgtctt catcttcccc ccaaagccca
     1501 aggatgtgct caccattact ctgactccta aggtcacgtg tgttgtggta gacatcagca
     1561 aggatgatcc cgaggtccag ttcagctggt ttgtagatga tgtggaggtg cacacagctc
     1621 agacgcaacc ccgggaggag cagttcaaca gcactttccg ctcagtcagt gaacttccca
     1681 tcatgcacca ggactggctc aatggcaagg agttcaaatg cagggtcaac agtgcagctt
     1741 tccctgcccc catcgagaaa accatctcca aaaccaaagg cagaccgaag gctccacagg
     1801 tgtacaccat tccacctccc aaggagcaga tggccaagga taaagtcagt ctgacctgca
     1861 tgataacaga cttcttccct gaagacatta ctgtggagtg gcagtggaat gggcagccag
     1921 cggagaacta caagaacact cagcccatca tggacacaga tggctcttac ttcgtctaca
     1981 gcaagctcaa tgtgcagaag agcaactggg aggcaggaaa tactttcacc tgctctgtgt
     2041 tacatgaggg cctgcacaac caccatactg agaagagcct ctcccactct cctggtaaat
     2101 aaacagctag cggttccaac cttggttccc ctctcccttt ccccccccta acgttactgg
     2161 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     2221 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     2281 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     2341 agttcctctg ggagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     2401 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     2461 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     2521 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     2581 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     2641 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     2701 agcttgccac aacccgggat cctctagagt cgacatggcc aagttgacca gtgccgttcc
     2761 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     2821 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     2881 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     2941 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     3001 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     3061 cgacccggcc ggcaactgcg tgcactttgt ggcagaggag caggactgag cggccgcaat
     3121 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3181 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3241 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3301 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3361 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3421 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3481 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3541 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3601 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3661 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcagat cccccgacct
     3721 cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3781 gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat
     3841 ctaagcttca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4801 ataccgcgtg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4861 agcgccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5641 ccccgaaaag tgccacctga agatctatac attgaatcaa tattggcaat tagccatatt
     5701 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     5761 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
//
