LOCUS       TGEX-HC-rbG-Zeo         5807 bp    DNA     circular
DEFINITION  Rabbit IgG TGEX Expression Vector (v7)
ACCESSION   
VERSION     .0
KEYWORDS    .
COMMENT     FT color
            FT join(612..1000,2125..2698)
            FT /number="8"
            FT color
            FT join(5..585,607..611,1104..1127,3115..3703,3719..3817)
            FT /number="17"
            FT color
            FT join(1041..1103,1128..2102)
            FT /number="14"
            FT color
            FT 2731..3106
            FT /number="20"
            FT color
            FT 3891..4510
            FT /number="2"
            FT color
            FT 4665..5525
            FT /number="10"
            FT color
            FT 5567..5595
            FT /number="1"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /note
     misc_feature    603
                     /gene
                     /label=Start
                     /note
     misc_feature    612..1000
                     /gene
                     /label=TPL
                     /note="including partial first intron with enhancer"
     primer_bind     974..995
                     /gene
                     /label=tgex-S3
                     /note
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
                     /color="14"
     misc_feature    2125..2698
                     /gene
                     /label=IRES
                     /note
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_feature    2097..2102
                     /gene
                     /label=Stop
                     /note
     CDS             1128..2099
                     /codon_start=1
                     /translation="GQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSG
                     TLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTCSK
                     PTCPPPELLGGPSVFIFPPKPKDTLMISRTPEVTCVVVDVSQDDPEVQFTWYINNEQV
                     RTARPPLREQQFNSTIRVVSTLPIAHQDWLRGKEFKCKVHNKALPAPIEKTISKARGQ
                     PLEPKVYTMGPPREELSSRSVSLTCMINGFYPSDISVEWEKNGKAEDNYKTTPAVLDS
                     DGSYFLYSKLSVPTSEWQRGDVFTCSVMHEALHNHYTQKSISRSPGK*"
                     /gene
                     /label=rbIgG
                     /note
                     /color="14"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    2103..2108
                     /db_xref="REBASE:NheI"
     misc_feature    3115..3703
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
                     /color="17"
     primer_bind     complement(2171..2190)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2635..2641
                     /gene
                     /label=A7
                     /note
     CDS             2732..3106
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene
                     /label=Zeo
                     /note
     misc_binding    3107..3114
                     /db_xref="REBASE:NotI"
     polyA_signal    3719..3817
                     /gene
                     /label=BGpA
                     /note
     rep_origin      complement(3891..4510)
                     /gene="pBR322 origin"
                     /label=pMB1
                     /note
     CDS             complement(4665..5525)
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
                     /product="TEM1 beta-lactamase"
     promoter        complement(5567..5595)
                     /gene
                     /label=AmpR_promoter
                     /note
     misc_binding    5658..5663
                     /db_xref="REBASE:BglII"
     misc_binding    3834..3839
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcaggg caacctaagg
     1141 ctccgtcagt cttcccactg gccccctgct gcggggacac acccagctcc acggtgaccc
     1201 tgggctgcct ggtcaaaggc tacctcccgg agccagtgac cgtgacctgg aactcgggca
     1261 ccctcaccaa tggggtacgc accttcccgt ccgtccggca gtcctcaggc ctctactcgc
     1321 tgagcagcgt ggtgagcgtg acctcaagca gccagcccgt cacctgcaac gtggcccacc
     1381 cagccaccaa caccaaagtg gacaagaccg ttgcgccctc gacatgcagc aagcccacgt
     1441 gcccaccccc tgaactcctg gggggaccgt ctgtcttcat cttcccccca aaacccaagg
     1501 acaccctcat gatctcacgc acccccgagg tcacatgcgt ggtggtggac gtgagccagg
     1561 atgaccccga ggtgcagttc acatggtaca taaacaacga gcaggtgcgc accgcccggc
     1621 cgccgctacg ggagcagcag ttcaacagca cgatccgcgt ggtcagcacc ctccccatcg
     1681 cgcaccagga ctggctgagg ggcaaggagt tcaagtgcaa agtccacaac aaggcactcc
     1741 cggcccccat cgagaaaacc atctccaaag ccagagggca gcccctggag ccgaaggtct
     1801 acaccatggg ccctccccgg gaggagctga gcagcaggtc ggtcagcctg acctgcatga
     1861 tcaacggctt ctacccttcc gacatctcgg tggagtggga gaagaacggg aaggcagagg
     1921 acaactacaa gaccacgccg gccgtgctgg acagcgacgg ctcctacttc ctctacagca
     1981 agctctcagt gcccacgagt gagtggcagc ggggcgacgt cttcacctgc tccgtgatgc
     2041 acgaggcctt gcacaaccac tacacgcaga agtccatctc ccgctctccg ggtaaataaa
     2101 cagctagcgg ttccaacctt ggttcccctc tccctttccc ccccctaacg ttactggccg
     2161 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2221 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2281 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2341 tcctctggga gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2401 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2461 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2521 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2581 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2641 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     2701 ttgccacaac ccgggatcct ctagagtcga catggccaag ttgaccagtg ccgttccggt
     2761 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     2821 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     2881 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     2941 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     3001 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     3061 cccggccggc aactgcgtgc actttgtggc agaggagcag gactgagcgg ccgcaatcaa
     3121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     3541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcagatccc ccgacctcga
     3721 cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     3781 tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct cggagatcta
     3841 agcttcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4801 ccgcgtgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagc
     4861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5641 cgaaaagtgc cacctgaaga tctatacatt gaatcaatat tggcaattag ccatattagt
     5701 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
     5761 tcataatatg tacatttata ttggctcatg tccaatatga ccgccat
//
