LOCUS       TGEX-HC                 4313 bp    DNA     circular
DEFINITION  Antibody expression vector TGEX-HC, complete sequence
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..608,609..611,2225..2323)
            FT /number="17"
            FT color
            FT 2397..3016
            FT /number="2"
            FT color
            FT 3171..4031
            FT /number="10"
            FT color
            FT 4073..4101
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT 1025..1050
            FT /number="20"
            FT color
            FT join(1051..1096,1182..1191,1192,1212..1548,1549..1878,
            FT 1879..2194)
            FT /number="14"
            FT color
            FT join(1209..1211,2195,2196..2201)
            FT /number="30"
            FT color
            FT 1097..1181
            FT /number="21"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_promoter
     misc_feature    603
                     /label=Start
                     /note="Transcription start"
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_feature    1025..1192
                     /label=IGHV1-2
                     /note="Partial human mRNA encoding leader sequence"
     CDS             1051..1096
                     /codon_start=1
                     /translation="MDWTWRILFLVAAAT"
     misc_feature    1097..1098
                     /label=SD
     misc_feature    1180..1181
                     /label=SA
     misc_binding    1182..1187
                     /db_xref="REBASE:BssHII"
     CDS             1184..1192
                     /codon_start=1
                     /translation="AHS"
     misc_feature    1193
                     /label=VH_Start
     misc_binding    1202..1207
                     /db_xref="REBASE:BsmBI"
     CDS             1209..1211
                     /codon_start=1
                     /translation="A"
     CDS             1212..2201
                     /codon_start=1
                     /translation="STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC
                     DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWY
                     VDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTI
                     SKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTT
                     PPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*"
                     /label=huG1
     misc_binding    2202..2209
                     /db_xref="REBASE:NotI"
     polyA_signal    2225..2323
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(2259..2282)
                     /label=tgex-R2
     misc_binding    2340..2345
                     /db_xref="REBASE:BglII"
     misc_binding    2346..2351
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(2397..3016)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2853)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(3171..4031)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     misc_binding    complement(3311..3316)
                     /db_xref="REBASE:BsaI"
     promoter        complement(4073..4101)
                     /label=AmpR_Promoter
     misc_binding    4164..4169
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcatcccc tgagagctcc gttcctcacc atggactgga cctggagaat cctcttcttg
     1081 gtggcagcag ccacaggtaa gaggctccct agtcccagtg atgagaaaga gattgagtcc
     1141 agtccaggga gatctcatcc acttctgtgt tctctccaca ggcgcgcact cccaggtgca
     1201 ccgtctctgc atccaccaag ggcccatcgg tcttccccct ggcaccctcc tccaagagca
     1261 cctctggggg cacagcggcc ctgggctgcc tggtcaagga ctacttcccc gaaccggtga
     1321 cggtgtcgtg gaactcaggc gccctgacca gcggcgtgca caccttcccg gctgtcctac
     1381 agtcctcagg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca
     1441 cccagaccta catctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagaaag
     1501 ttgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccagca cctgaactcc
     1561 tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc
     1621 ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt
     1681 tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc
     1741 agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga
     1801 atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa
     1861 ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg cccccatccc
     1921 gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca
     1981 gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc
     2041 ctcccgtgct ggactccgac ggctccttct tcctctatag caagctcacc gtggacaaga
     2101 gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc
     2161 actacacgca gaagagcctc tccctgtctc cgggtaaatg agcggccgca gatcccccga
     2221 cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     2281 tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga
     2341 gatctaagct tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3361 ggaagcgccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4141 tttccccgaa aagtgccacc tgaagatcta tacattgaat caatattggc aattagccat
     4201 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     4261 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc cat
//
