LOCUS       TGEX-LC-hL2-Zeo         5156 bp    DNA     circular
DEFINITION  Human Lambda 2 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,2464..3052,3068..3166)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,1446..1449,4916..4944)
            FT /number="1"
            FT color
            FT 2081..2455
            FT /number="20"
            FT color
            FT join(1041..1099,1132..1445,1450..1451)
            FT /number="14"
            FT translated
            FT 2081..2455
            FT /frame="2"
            FT color
            FT 3240..3859
            FT /number="2"
            FT color
            FT join(1474..1484,1487..1986,1988,1991..2047)
            FT /number="7"
            FT color
            FT join(4014..4154,4156..4874)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..1449
                     /codon_start=1
                     /translation="GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
                     DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP
                     TECS*"
                     /gene
                     /label=hCL2*01
                     /note
     misc_feature    1446..1451
                     /gene
                     /label=Stop
                     /note
     misc_binding    1452..1457
                     /db_xref="REBASE:NheI"
     misc_feature    1474..2047
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1520..1539)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    1984..1990
                     /label=A7
                     /note
     misc_binding    2069..2074
                     /db_xref="REBASE:XbaI"
     misc_binding    2075..2080
                     /db_xref="REBASE:SalI"
     CDS             2081..2455
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    2456..2463
                     /db_xref="REBASE:NotI"
     misc_feature    2464..3052
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3068..3166
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3183..3188
                     /db_xref="REBASE:BglII"
     misc_binding    3189..3194
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3240..3859)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(3696)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4014..4874)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(4916..4944)
                     /label=AmpR_Promoter
     misc_binding    5007..5012
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcaggg cagcccaagg
     1141 ctgccccctc ggtcactctg ttcccgccct cctctgagga gcttcaagcc aacaaggcca
     1201 cactggtgtg tctcataagt gacttctacc cgggagccgt gacagtggct tggaaagcag
     1261 atagcagccc cgtcaaggcg ggagtggaaa ccaccacacc ctccaaacaa agcaacaaca
     1321 agtacgcggc cagcagctat ctgagcctga cgcctgagca gtggaagtcc cacagaagct
     1381 acagctgcca ggtcacgcat gaagggagca ccgtggagaa gacagtggcc cctacagaat
     1441 gttcataaac agctagcggt tccaaccttg gttcccctct ccctttcccc cccctaacgt
     1501 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     1561 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     1621 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     1681 ggaagcagtt cctctgggag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     1741 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     1801 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     1861 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     1921 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     1981 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     2041 atgataagct tgccacaacc cgggatcctc tagagtcgac atggccaagt tgaccagtgc
     2101 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     2161 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     2221 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     2281 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     2341 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     2401 cctgcgcgac ccggccggca actgcgtgca ctttgtggca gaggagcagg actgagcggc
     2461 cgcaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2521 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2581 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2641 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2701 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2761 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2821 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     2881 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2941 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3001 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcagatcccc
     3061 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     3121 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
     3181 ggagatctaa gcttcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4141 gcaatgatac cgcgtgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4201 gccggaagcg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4981 acatttcccc gaaaagtgcc acctgaagat ctatacattg aatcaatatt ggcaattagc
     5041 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     5101 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccat
//
