LOCUS       TGEX-LC-mL2-Zeo         5153 bp    DNA     circular
DEFINITION  Murine BALB/c Lambda 2 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,2461..3049,3065..3163)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,4913..4941)
            FT /number="1"
            FT color
            FT 2078..2452
            FT /number="20"
            FT color
            FT join(1041..1099,1132..1448)
            FT /number="14"
            FT translated
            FT 2078..2452
            FT /frame="2"
            FT color
            FT 3237..3856
            FT /number="2"
            FT color
            FT join(1471..1481,1484..1983,1985,1988..2044)
            FT /number="7"
            FT color
            FT join(4011..4151,4153..4871)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..1445
                     /codon_start=1
                     /translation="GQPKSTPTLTVFPPSSEELKENKATLVCLISNFSPSGVTVAWKA
                     NGTPITQGVDTSNPTKEGNKFMASSFLHLTSDQWRSHNSFTCQVTHEGDTVEKSLSPA
                     ECL*"
                     /label=mL2*01
     misc_feature    1443..1448
                     /gene
                     /label=Stop
                     /note
     misc_binding    1449..1454
                     /db_xref="REBASE:NheI"
     misc_feature    1471..2044
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1517..1536)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    1981..1987
                     /label=A7
                     /note
     misc_binding    2066..2071
                     /db_xref="REBASE:XbaI"
     misc_binding    2072..2077
                     /db_xref="REBASE:SalI"
     CDS             2078..2452
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    2453..2460
                     /db_xref="REBASE:NotI"
     misc_feature    2461..3049
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3065..3163
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3180..3185
                     /db_xref="REBASE:BglII"
     misc_binding    3186..3191
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3237..3856)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(3693)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4011..4871)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(4913..4941)
                     /label=AmpR_Promoter
     misc_binding    5004..5009
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcaggg cagcccaagt
     1141 ccactcccac tctcaccgtg tttccacctt cctctgagga gctcaaggaa aacaaagcca
     1201 cactggtgtg tctgatttcc aacttttccc cgagtggtgt gacagtggcc tggaaggcaa
     1261 atggtacacc tatcacccag ggtgtggaca cttcaaatcc caccaaagag ggcaacaagt
     1321 tcatggccag cagcttccta catttgacat cggaccagtg gagatctcac aacagtttta
     1381 cctgtcaagt tacacatgaa ggggacactg tggagaagag tctgtctcct gcagaatgtc
     1441 tctaaacagc tagcggttcc aaccttggtt cccctctccc tttccccccc ctaacgttac
     1501 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     1561 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     1621 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     1681 agcagttcct ctgggagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     1741 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     1801 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     1861 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     1921 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     1981 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     2041 ataagcttgc cacaacccgg gatcctctag agtcgacatg gccaagttga ccagtgccgt
     2101 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     2161 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     2221 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     2281 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     2341 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     2401 gcgcgacccg gccggcaact gcgtgcactt tgtggcagag gagcaggact gagcggccgc
     2461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2821 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     2881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca gatcccccga
     3061 cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3121 tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga
     3181 gatctaagct tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3661 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4141 atgataccgc gtgacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4201 ggaagcgccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4981 tttccccgaa aagtgccacc tgaagatcta tacattgaat caatattggc aattagccat
     5041 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     5101 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc cat
//
