LOCUS       TGEX-LC-rbKb4-Ze        5150 bp    DNA     circular
DEFINITION  Rabbit Kappa TGEX Expression Vector (v7)
ACCESSION   
VERSION     .0
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,1104..1127,2458..3046,3062..3160)
            FT /number="17"
            FT color
            FT join(612..1000,1468..2041)
            FT /number="8"
            FT color
            FT join(1041..1103,1128..1442)
            FT /number="30"
            FT color
            FT 2075..2449
            FT /number="20"
            FT color
            FT 3234..3853
            FT /number="2"
            FT color
            FT 4008..4868
            FT /number="10"
            FT color
            FT 4910..4938
            FT /number="1"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /gene
                     /label=CMV_promoter
                     /note
     misc_feature    603
                     /gene
                     /label=Start
                     /note
     misc_feature    612..1000
                     /gene
                     /label=TPL
                     /note="including partial first intron with enhancer"
     primer_bind     974..995
                     /gene
                     /label=tgex-S3
                     /note
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     CDS             1128..1442
                     /codon_start=1
                     /translation="GDPVAPTVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDG
                     TTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRG
                     DC*"
                     /gene
                     /label=rbKb4
                     /note
     misc_feature    1440..1445
                     /gene
                     /label=Stop
                     /note
     misc_binding    1446..1451
                     /db_xref="REBASE:NheI"
     misc_feature    1468..2041
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1514..1533)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    1978..1984
                     /gene
                     /label=A7
                     /note
     CDS             2075..2449
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene
                     /label=Zeo
                     /note
     misc_binding    2450..2457
                     /db_xref="REBASE:NotI"
     misc_feature    2458..3046
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
                     /color="17"
     polyA_signal    3062..3160
                     /gene
                     /label=BGpA
                     /note
     misc_binding    3177..3182
                     /db_xref="REBASE:BglII"
     rep_origin      complement(3234..3853)
                     /gene="pBR322 origin"
                     /label=pMB1
                     /note
     misc_binding    5001..5006
                     /db_xref="REBASE:BglII"
     CDS             complement(4008..4868)
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
                     /product="TEM1 beta-lactamase"
     promoter        complement(4910..4938)
                     /gene
                     /label=AmpR_promoter
                     /note
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagga gatccagttg
     1141 cacctactgt cctcatcttc ccaccagctg ctgatcaggt ggcaactgga acagtcacca
     1201 tcgtgtgtgt ggcgaataaa tactttcccg atgtcaccgt cacctgggag gtggatggca
     1261 ccacccaaac aactggcatc gagaacagta aaacaccgca gaactctgca gattgtacct
     1321 acaacctcag cagcactctg acactgacca gcacacagta caacagccac aaagagtaca
     1381 cctgcaaggt gacccagggc acgacctcag tcgtccagag cttcaatcgt ggtgactgtt
     1441 aaacagctag cggttccaac cttggttccc ctctcccttt ccccccccta acgttactgg
     1501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     1561 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     1621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     1681 agttcctctg ggagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     1801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     1861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     1921 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     1981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     2041 agcttgccac aacccgggat cctctagagt cgacatggcc aagttgacca gtgccgttcc
     2101 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     2161 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     2221 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     2281 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     2341 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     2401 cgacccggcc ggcaactgcg tgcactttgt ggcagaggag caggactgag cggccgcaat
     2461 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2521 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2581 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2641 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2701 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2761 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2821 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     2881 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2941 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3001 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcagat cccccgacct
     3061 cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3121 gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat
     3181 ctaagcttca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4141 ataccgcgtg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4201 agcgccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4981 ccccgaaaag tgccacctga agatctatac attgaatcaa tattggcaat tagccatatt
     5041 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     5101 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
//
