LOCUS       TGEX-LC                 3787 bp    DNA     circular
DEFINITION  Antibody expression vector TGEX-LC, complete sequence
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..608,609..611,1699..1797)
            FT /number="17"
            FT color
            FT 1871..2490
            FT /number="2"
            FT color
            FT join(2645..2785,2787..3505)
            FT /number="10"
            FT color
            FT 3547..3575
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT 1311..1634
            FT /number="12"
            FT color
            FT join(1025..1042,1092..1278)
            FT /number="21"
            FT color
            FT join(1043..1091,1279..1289)
            FT /number="14"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_promoter
     misc_feature    603
                     /label=Start
                     /note="Transcription start"
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     misc_feature    1025..1289
                     /label=IGKV3-20
                     /note="Partial human mRNA encoding leader sequence"
     CDS             join(1043..1091,1279..1289)
                     /codon_start=1
                     /translation="METPAQLLFLLLLWLPDTSG"
     misc_feature    1092..1093
                     /label=SD
     misc_feature    1277..1278
                     /label=SA
     misc_binding    1284..1289
                     /db_xref="REBASE:BspEI"
     misc_feature    1290
                     /label=VL_Start
     misc_binding    1304..1309
                     /db_xref="REBASE:BsaI"
     CDS             1311..1313
                     /codon_start=1
                     /translation="R"
     CDS             1312..1634
                     /codon_start=3
                     /translation="TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDN
                     ALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN
                     RGEC*"
                     /label=hCK
     misc_binding    1676..1683
                     /db_xref="REBASE:NotI"
     polyA_signal    1699..1797
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(1733..1756)
                     /label=tgex-R2
     misc_binding    1814..1819
                     /db_xref="REBASE:BglII"
     misc_binding    1820..1825
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(1871..2490)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2327)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(2645..3505)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(3547..3575)
                     /label=AmpR_Promoter
     misc_binding    3638..3643
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attctaggac ccagagggaa ccatggaaac cccagcgcag cttctcttcc tcctgctact
     1081 ctggctccca ggtgagggga acatgggatg gttttgcatg tcagtgaaaa ccctctcaag
     1141 tcctgttacc tggcaactct gctcagtcaa tacaataatt aaagctcaat ataaagcaat
     1201 aattctggct cttctgggaa gacaatgggt ttgatttaga ttacatgggt gacttttctg
     1261 ttttatttcc aatctcagat acctccggag aacgtgagta gacggtctcg cgaactgtgg
     1321 ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct ggaactgcct
     1381 ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag tggaaggtgg
     1441 ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac agcaaggaca
     1501 gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag aaacacaaag
     1561 tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag agcttcaaca
     1621 ggggagagtg ttagagggag cctgcaggat tccgccccat tggccgaaca cacacgcggc
     1681 cgcagatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     1741 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     1801 cgagatccct cggagatcta agcttcatgt gagcaaaagg ccagcaaaag gccaggaacc
     1861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2281 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2341 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2401 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2461 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2521 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2581 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2641 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2701 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2761 gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat ttatcagcaa
     2821 taaaccagcc agccggaagc gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2881 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2941 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3001 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3061 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3121 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3181 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3241 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3301 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3361 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3421 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3481 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3541 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3601 gggttccgcg cacatttccc cgaaaagtgc cacctgaaga tctatacatt gaatcaatat
     3661 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     3721 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     3781 ccgccat
//

