LOCUS       TGEX-SCblue             4323 bp    DNA     circular
DEFINITION  Antibody expression vector TGEX-SCblue, complete sequence
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     Antibody expression vector TGEX-SCblue, complete sequence
            FT color
            FT join(5..585,607..608,609..611,2235..2333)
            FT /number="17"
            FT color
            FT 2407..3026
            FT /number="2"
            FT color
            FT join(3181..3321,3323..4041)
            FT /number="10"
            FT color
            FT join(1209..1229,4083..4111,1244..1249,1268..1270,1271..1272,
            FT 1273)
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1043..1102,1498..1512,1513..1558,1559..1888,1889..2211)
            FT /number="14"
            FT color
            FT 1280..1300
            FT /number="19"
            FT color
            FT join(1103..1187,1301..1317)
            FT /number="27"
            FT color
            FT 1188..1208
            FT /number="20"
            FT translated
            FT join(1318..1488,1498..1512)
            FT /frame="1"
            FT color
            FT join(5..585,607..608,609..611,2235..2333)
            FT /number="17"
            FT color
            FT 2407..3026
            FT /number="2"
            FT color
            FT join(3181..3321,3323..4041)
            FT /number="10"
            FT color
            FT join(1209..1229,4083..4111,1244..1249,1268..1270,1271..1272,
            FT 1273)
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT color
            FT join(1043..1102,1498..1512,1513..1558,1559..1888,1889..2211)
            FT /number="14"
            FT color
            FT 1280..1300
            FT /number="19"
            FT color
            FT join(1103..1187,1301..1317)
            FT /number="27"
            FT color
            FT 1188..1208
            FT /number="20"
            FT translated
            FT join(1318..1488,1498..1512)
            FT /frame="1"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_promoter
     misc_feature    603
                     /label=Start
                     /note="Transcription start"
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1043..1105
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1087..1099
                     /db_xref="REBASE:SfiI"
     misc_binding    1188..1208
                     /label=operator_O3
     misc_binding    1209..1229
                     /label=CAP_binding
     -35_signal      1244..1249
     -10_signal      1268..1273
     misc_binding    1280..1300
                     /label=operator_O1
     misc_binding    1498..1510
                     /db_xref="REBASE:SfiI"
     CDS             1513..2211
                     /codon_start=1
                     /translation="EPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV
                     TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS
                     DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH
                     NHYTQKSLSLSPGK*"
                     /label=IgG1-Fc
     misc_difference 1526
                     /note="G>C mutation to convert Cys into Ser."
     misc_binding    2212..2219
                     /db_xref="REBASE:NotI"
     polyA_signal    2235..2333
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(2269..2292)
                     /label=tgex-R2
     misc_binding    2350..2355
                     /db_xref="REBASE:BglII"
     misc_binding    2356..2361
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(2407..3026)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2863)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(3181..4041)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(4083..4111)
                     /label=AmpR_Promoter
     misc_binding    4174..4179
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg gggatatcca ccatggagac agacacactc ctgctatggg tactgctgct
     1081 cttagcggcc cagccggcca tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1141 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1201 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     1261 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1321 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1381 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1441 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaattaaaa tagatagggc
     1501 ccgggaggcc ccgagcccaa atcttctgac aaaactcaca catgcccacc gtgcccagca
     1561 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc
     1621 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct
     1681 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg
     1741 cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag
     1801 gactggctga atggcaagga gtacaagtgc aaggtgtcca acaaagccct cccagccccc
     1861 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg
     1921 cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
     1981 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac
     2041 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctatag caagctcacc
     2101 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct
     2161 ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaatg agcggccgca
     2221 gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     2281 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag
     2341 atccctcgga gatctaagct tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2821 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3301 cagtgctgca atgataccgc gtgacccacg ctcaccggct ccagatttat cagcaataaa
     3361 ccagccagcc ggaagcgccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4141 tccgcgcaca tttccccgaa aagtgccacc tgaagatcta tacattgaat caatattggc
     4201 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     4261 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     4321 cat
//

