LOCUS       TGEX-eGFP-Zeo           5455 bp    DNA     circular
DEFINITION  eGFP TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,2763..3351,3367..3465)
            FT /number="17"
            FT translated
            FT 1025
            FT /frame="3"
            FT color
            FT 1031..1750
            FT /number="15"
            FT color
            FT 612..1000
            FT /number="8"
            FT translated
            FT 2380..2754
            FT /frame="1"
            FT color
            FT 5215..5243
            FT /number="1"
            FT color
            FT 3539..4158
            FT /number="2"
            FT color
            FT join(1773..1783,1786..2285,2287,2290..2346)
            FT /number="7"
            FT color
            FT join(4313..4453,4455..5173)
            FT /number="10"
            FT color
            FT 2380..2754
            FT /number="20"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     gene            1031..1750
                     /gene="mgfp"
     CDS             1031..1750
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYK*"
                     /label=eGFP
                     /db_xref="GI:1377912"
     misc_binding    1751..1756
                     /db_xref="REBASE:NheI"
     misc_feature    1773..2346
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1819..1838)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2283..2289
                     /label=A7
                     /note
     misc_binding    2368..2373
                     /db_xref="REBASE:XbaI"
     misc_binding    2374..2379
                     /db_xref="REBASE:SalI"
     CDS             2380..2754
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    2755..2762
                     /db_xref="REBASE:NotI"
     misc_feature    2763..3351
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3367..3465
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3482..3487
                     /db_xref="REBASE:BglII"
     misc_binding    3488..3493
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3539..4158)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(3995)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4313..5173)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5215..5243)
                     /label=AmpR_Promoter
     misc_binding    5306..5311
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcgccacc atggcgagca agggcgaaga actgtttacc ggcgtggtgc ccatcctggt
     1081 ggaactggat ggggatgtga acggccacaa gttcagcgtt agcggagaag gcgaaggcga
     1141 cgccacatac ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc
     1201 ttggcctaca ctggtcacca cactgacata cggcgtgcag tgcttcgcca gatatcccga
     1261 ccatatgaag cagcacgact tcttcaagag cgccatgcct gagggctacg tgcaagagcg
     1321 gaccatcttc tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg
     1381 cgacaccctg gtcaaccgga tcgagctgaa gggcatcgac ttcaaagagg acggcaacat
     1441 cctgggccac aagctggagt acaactacaa cagccacaag gtgtacatca ccgccgacaa
     1501 gcagaaaaac ggcatcaaag tgaacttcaa gacccggcac aacatcgagg acggctctgt
     1561 gcagctggcc gatcactacc agcagaacac acccatcgga gatggccctg tgctgctgcc
     1621 cgataaccac tacctgagca cacagagcgc cctgagcaag gaccccaacg agaagaggga
     1681 tcacatggtg ctgctggagt tcgtgaccgc cgctggcatc acacacggca tggatgagct
     1741 gtacaagtaa gctagcggtt ccaaccttgg ttcccctctc cctttccccc ccctaacgtt
     1801 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1861 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1921 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1981 gaagcagttc ctctgggagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     2041 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     2101 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     2161 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     2221 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     2281 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     2341 tgataagctt gccacaaccc gggatcctct agagtcgaca tggccaagtt gaccagtgcc
     2401 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     2461 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     2521 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     2581 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     2641 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     2701 ctgcgcgacc cggccggcaa ctgcgtgcac tttgtggcag aggagcagga ctgagcggcc
     2761 gcaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2821 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2881 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2941 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3001 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3061 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3121 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3181 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3241 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3301 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cagatccccc
     3361 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3421 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3481 gagatctaag cttcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4441 caatgatacc gcgtgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4501 ccggaagcgc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5281 catttccccg aaaagtgcca cctgaagatc tatacattga atcaatattg gcaattagcc
     5341 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     5401 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccat
//
