LOCUS       TGEX-eGFP               3884 bp    DNA     circular
DEFINITION  
ACCESSION   
VERSION     .5
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,1796..1894)
            FT /number="17"
            FT color
            FT 1968..2587
            FT /number="2"
            FT color
            FT 3644..3672
            FT /number="1"
            FT color
            FT 612..1000
            FT /number="8"
            FT translated
            FT 1025
            FT /frame="3"
            FT color
            FT join(2742..2882,2884..3602)
            FT /number="10"
            FT color
            FT 1031..1750
            FT /number="15"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_immearly_promoter
     CAAT_signal     540..544
     TATA_signal     573..577
     misc_feature    602
                     /label=mRNA
     misc_feature    612..1000
                     /label=TPL
     primer_bind     974..995
                     /label=tgex_S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     gene            1031..1750
                     /gene="mgfp"
     CDS             1031..1750
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN
                     GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYK*"
                     /label=eGFP
                     /db_xref="GI:1377912"
     misc_binding    1773..1780
                     /db_xref="REBASE:NotI"
     misc_binding    1774..1779
                     /db_xref="REBASE:EagI"
     polyA_signal    1796..1894
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(1830..1853)
                     /label=tgex_R2
     misc_binding    1911..1916
                     /db_xref="REBASE:BglII"
     misc_binding    1917..1922
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(1968..2587)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(2424)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(2742..3602)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(3644..3672)
                     /label=AmpR_Promoter
     misc_binding    3735..3740
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcgccacc atggcgagca agggcgaaga actgtttacc ggcgtggtgc ccatcctggt
     1081 ggaactggat ggggatgtga acggccacaa gttcagcgtt agcggagaag gcgaaggcga
     1141 cgccacatac ggaaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc
     1201 ttggcctaca ctggtcacca cactgacata cggcgtgcag tgcttcgcca gatatcccga
     1261 ccatatgaag cagcacgact tcttcaagag cgccatgcct gagggctacg tgcaagagcg
     1321 gaccatcttc tttaaggacg acggcaacta caagaccagg gccgaagtga agttcgaggg
     1381 cgacaccctg gtcaaccgga tcgagctgaa gggcatcgac ttcaaagagg acggcaacat
     1441 cctgggccac aagctggagt acaactacaa cagccacaag gtgtacatca ccgccgacaa
     1501 gcagaaaaac ggcatcaaag tgaacttcaa gacccggcac aacatcgagg acggctctgt
     1561 gcagctggcc gatcactacc agcagaacac acccatcgga gatggccctg tgctgctgcc
     1621 cgataaccac tacctgagca cacagagcgc cctgagcaag gaccccaacg agaagaggga
     1681 tcacatggtg ctgctggagt tcgtgaccgc cgctggcatc acacacggca tggatgagct
     1741 gtacaagtaa gctagcggtt ccaaccttgg ttgcggccgc agatcccccg acctcgacct
     1801 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     1861 cactcggaag gacatatggg agggcaaatc atttggtcga gatccctcgg agatctaagc
     1921 ttcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2881 cgtgacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagcgcc
     2941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3721 aaagtgccac ctgaagatct atacattgaa tcaatattgg caattagcca tattagtcat
     3781 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
     3841 taatatgtac atttatattg gctcatgtcc aatatgaccg ccat
//
