LOCUS fADL-1 7982 bp DNA circular DEFINITION Filamentous phage cloning vector fADL-1, complete sequence. ACCESSION VERSION .2 KEYWORDS . COMMENT Filamentous phage cloning vector constructed by splicing the kanamycin resistance determinant of Tn903 into the minus-strand origin of bacteriphage fd. Transduce kanamycin resistance into infected host cell. Double-stranded RF copy number is about 60 copies in stationary-phase cells. Yield of physical particles is about 5e11 virions/ml. Make very small plaques. Display on gene III protein up to 100% with limited polyphage production. FEATURES Location/Qualifiers CDS join(7581..7982,1..831) /codon_start=1 /transl_table=11 /product="replication protein pII" /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM TSYQKEQLKVLSNPDLLEFASGLVRFEARIETRYLKSFGLPLNLFDAIRFASDYNRQG KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV DFSSQRPDWYNEPVLKIA*" CDS 496..831 /codon_start=1 /transl_table=11 /product="replication protein pX" /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW YNEPVLKIA" CDS 843..1106 /codon_start=1 /transl_table=11 /product="cytosolic single-stranded DNA binding protein pV" /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK" CDS 1108..1209 /codon_start=1 /transl_table=11 /product="minor coat protein pVII" /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR" CDS 1206..1304 /codon_start=1 /transl_table=11 /product="minor coat protein pIX" /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS" CDS 1301..1522 /codon_start=1 /transl_table=11 /product="major coat protein pVIII precursor" /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFDSLQASATEY IGYAWAMVVVIVGATIGIKLFKKFTSKAS" sig_peptide 1301..1369 CDS 2901..3239 /codon_start=1 /transl_table=11 /product="minor coat protein pVI" /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ KIVSYLDWDK*" CDS 3242..4288 /codon_start=1 /transl_table=11 /product="morphogenetic protein pI" /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL YSLVTGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSITYIDLCTV SIKKGNSNEIVKCN*" CDS 4266..5546 /codon_start=1 /transl_table=11 /product="morphogenetic protein IV" /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQTGE SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNSQDYI DELPSSDIQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS SNVLSVDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTVERQNVGISMSVFPVAMAGGNIV LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*" misc_feature 5545..5622 /note="morphogenetic protein signal sequence; promotes packaging of circular single plus strands into progeny virions; other site" rep_origin join(5669..5690,7220..7331) /note="minus-strand origin, disrupted by Tn903-derived insert; functionally inactive; minus strand initiated randomly by endogenous bacterial Okazaki fragment priming apparatus" rep_origin 7340..7479 /note="plus strand origin" misc_feature 7355^7356 /note="pII replication protein nicks plus strand to initiate rolling circle replication" misc_binding 6020..6025 /db_xref="REBASE:HindIII" CDS complement(6274..7089) /gene="aph(3')-Ia" /note="Confer resistance to kanamycin" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" CDS 1586..1648 /codon_start=1 /translation="MKYLLPTAAAGLLLLAAQPAM" /label=PelB CDS 1678..2898 /codon_start=1 /product="morphogenetic protein III" /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY VFSTFANILRNKES*" /label=g3p misc_binding 1633..1645 /db_xref="REBASE:SfiI" misc_binding 1654..1666 /db_xref="REBASE:SfiI" misc_binding 1634..1644 /db_xref="REBASE:BglI" misc_binding 1655..1665 /db_xref="REBASE:BglI" misc_binding 2265..2270 /db_xref="REBASE:BamHI" misc_binding 1607..1614 /db_xref="REBASE:NotI" misc_binding 1671..1676 /db_xref="REBASE:XbaI" misc_binding 1565..1570 /db_xref="REBASE:SalI" ORIGIN 1 aacgctacta ccattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 181 gttgcatatt taaaacatgt tgaactacag caccagattc agcaattaag ctctaagcca 241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactgtctaa tcctgacctg 301 ttggaatttg cttccggtct ggttcgcttt gaggctcgaa ttgaaacgcg atatttgaag 361 tctttcgggc ttcctcttaa tctttttgat gcaattcgct ttgcttctga ctataataga 421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 541 aaacatttta caattacccc ctctggcaaa acttcctttg caaaagcctc tcgctatttt 601 ggtttctatc gtcgtctggt taatgagggt tatgatagtg ttgctcttac catgcctcgt 661 aattcctttt ggcgttatgt atctgcatta gttgagtgtg gtattcctaa atctcaattg 721 atgaatcttt ccacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 781 tcctcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 841 aaatgattaa agttgaaatt aaaccatctc aagcgcaatt tactacccgt tctggtgttt 901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 961 aatatccggt gcttgtcaag attactctcg acgaaggtca gccagcgtat gcgcctggtc 1021 tgtacaccgt gcatctgtcc tcgttcaaag ttggtcagtt cggttctctt atgattgacc 1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1321 caaagcctcc gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1381 cgatcccgca aaagcggcct ttgactccct gcaagcctca gcgaccgaat atatcggtta 1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1561 ttttgtcgac taacgagggc aaatcatgaa atacctattg cctacggcgg ccgctggatt 1621 gttattactc gcggcccagc cggccatgct agtggcccgg gaggcctgtc tctagaagcc 1681 gaaactgttg aaagttgttt agcaaaacct catacagaaa attcatttac taacgtctgg 1741 aaagacgaca aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca 1801 ggcgttgtgg tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg 1861 cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt 1921 tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact 1981 tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct 2041 aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc 2101 cgaaataggc agggtgcatt aactgtttat acgggcactg ttactcaagg cactgacccc 2161 gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg 2221 aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt 2281 gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct 2341 ggtggtggtt ctggtggcgg ctctgagggt ggcggctctg agggtggcgg ttctgagggt 2401 ggcggctctg agggtggcgg ttccggtggc ggctccggtt ccggtgattt tgattatgaa 2461 aaaatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa cgcgctacag 2521 tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc tatcgatggt 2581 ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga ttttgctggc 2641 tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat gaataatttc 2701 cgtcaatatt taccttcttt gcctcagtcg gttgaatgtc gcccttatgt ctttggcgct 2761 ggtaaaccat atgaattttc tattgattgt gacaaaataa acttattccg tggtgtcttt 2821 gcgtttcttt tatatgttgc cacctttatg tatgtatttt cgacgtttgc taacatactg 2881 cgtaataagg agtcttaatc atgccagttc ttttgggtat tccgttatta ttgcgtttcc 2941 tcggtttcct tctggtaact ttgttcggct atctgcttac tttccttaaa aagggcttcg 3001 gtaagatagc tattgctatt tcattgtttc ttgctcttat tattgggctt aactcaattc 3061 ttgtgggtta tctctctgat attagcgcac aattaccctc tgattttgtt cagggcgttc 3121 agttaattct cccgtctaat gcgcttccct gtttttatgt tattctctct gtaaaggctg 3181 ctattttcat ttttgacgtt aaacaaaaaa tcgtttctta tttggattgg gataaataaa 3241 tatggctgtt tattttgtaa ctggcaaatt aggctctgga aagacgctcg ttagcgttgg 3301 taagattcag gataaaattg tagctgggtg caaaatagca actaatcttg atttaaggct 3361 tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta gaataccgga 3421 taagccttct atttctgatt tgcttgctat tggtcgtggt aatgattcct acgacgaaaa 3481 taaaaacggt ttgcttgttc ttgatgaatg cggtacttgg tttaataccc gttcatggaa 3541 tgacaaggaa agacagccga ttattgattg gtttcttcat gctcgtaaat tgggatggga 3601 tattattttt cttgttcagg atttatctat tgttgataaa caggcgcgtt ctgcattagc 3661 tgaacacgtt gtttattgtc gccgtctgga cagaattact ttaccctttg tcggcacttt 3721 atattctctt gttactggct caaaaatgcc tctgcctaaa ttacatgttg gtgttgttaa 3781 atatggtgat tctcaattaa gccctactgt tgagcgttgg ctttatactg gtaagaattt 3841 atataacgca tatgacacta aacaggcttt ttccagtaat tatgattcag gtgtttattc 3901 atatttaacc ccttatttat cacacggtcg gtatttcaaa ccattaaatt taggtcagaa 3961 gatgaaatta actaaaatat atttgaaaaa gttttctcgc gttctttgtc ttgcgatagg 4021 atttgcatca gcatttacat atagttatat aacccaacct aagccggagg ttaaaaaggt 4081 agtctctcag acctatgatt ttgataaatt cactattgac tcttctcagc gtcttaatct 4141 aagctatcgc tatgttttca aggattctaa gggaaaatta attaatagcg acgatttaca 4201 gaagcaaggt tattccatca catatattga tttatgtact gtttcaatta aaaaaggtaa 4261 ttcaaatgaa attgttaaat gtaattaatt ttgttttctt gatgtttgtt tcatcatctt 4321 cttttgctca agtaattgaa atgaataatt cgcctctgcg cgatttcgtg acttggtatt 4381 caaagcaaac aggtgaatct gttattgtct cacctgatgt taaaggtaca gtgactgtat 4441 attcctctga cgttaagcct gaaaatttac gcaatttctt tatctctgtt ttacgtgcta 4501 ataattttga tatggttggc tcaattcctt ccataattca gaaatataac ccaaatagtc 4561 aggattatat tgatgaattg ccatcatctg atattcagga atatgatgat aattccgctc 4621 cttctggtgg tttctttgtt ccgcaaaatg ataatgttac tcaaacattt aaaattaata 4681 acgttcgcgc aaaggattta ataagggttg tagaattgtt tgttaaatct aatacatcta 4741 aatcctcaaa tgtattatct gttgatggtt ctaacttatt agtagttagc gcccctaaag 4801 atattttaga taaccttccg caatttcttt ctactgttga tttgccaact gaccagatat 4861 tgattgaagg attaattttc gaggttcagc aaggtgatgc tttagatttt tcctttgctg 4921 ctggctctca gcgcggcact gttgctggtg gtgttaatac tgaccgtcta acctctgttt 4981 tatcttctgc gggtggttcg ttcggtattt ttaacggcga tgttttaggg ctatcagttc 5041 gcgcattaaa gactaatagc cattcaaaaa tattgtctgt gcctcgtatt cttacgcttt 5101 caggtcagaa gggttctatt tctgttggcc agaatgtccc ttttattact ggtcgtgtaa 5161 ctggtgaatc tgccaatgta aataatccat ttcagacggt tgagcgtcaa aatgttggta 5221 tttctatgag tgtttttccc gttgcaatgg ctggcggtaa tattgtttta gatataacca 5281 gtaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact aatcaaagaa 5341 gtattgcgac aacggttaat ttgcgtgatg gtcagactct tttgctcggt ggcctcactg 5401 attacaaaaa cacttctcaa gattctggtg tgccgttcct gtctaaaatc cctttaatcg 5461 gcctcctgtt tagctcccgt tctgattcta acgaggaaag cacgttgtac gtgctcgtca 5521 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 5581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 5641 tcctttctcg ccacgttctc cggctttccc cgtcaagctc taaatcgggg gatctcggga 5701 aaagcgttgg tgaccaaagg tgccttttat catcacttta aaaataaaaa acaattactc 5761 agtgcctgtt ataagcagca attaattatg attgatgcct acatcacaac aaaaactgat 5821 ttaacaaatg gttggtctgc cttagaaagt atatttgaac attatcttga ttatattatt 5881 gataataata aaaaccttat ccctatccaa gaagtgatgc ctatcattgg ttggaatgaa 5941 cttgaaaaaa ttagccttga atacattact ggtaaggtaa acgccattgt cagcaaattg 6001 atccaagaga accaacttaa agcttatgat gatgatgtgc ttaaaaactt actcaatggc 6061 tggtttatgc atatcgcaat acatgcgaaa aacctaaaag agcttgccga taaaaaaggc 6121 caatttattg ctatttaccg cggcttttta ttgagcttga aagataaata aaatagatag 6181 gttttatttg aagctaaatc ttctttatcg taaaaaatgc cctcttgggt tatcaagagg 6241 gtcattatat ttcgcggaat aaaccaattc tgattagaaa aactcatcga gcatcaaatg 6301 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg 6361 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc 6421 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag 6481 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt 6541 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact 6601 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc 6661 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag 6721 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt 6781 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat 6841 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc 6901 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata 6961 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata 7021 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat 7081 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga 7141 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttt 7201 gttgacaaag ggaatcatag atccctttag ggttccgatt tagtgcttta cggcacctcg 7261 acctccaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 7321 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 7381 gaacaacact cacaactaac tcggcctatt cttttgattt ataaggattt ttgtcatttt 7441 ctgcttactg gttaaaaaat aagctgattt aacaaatatt taacgcgaaa tttaacaaaa 7501 cattaacgtt tacaatttaa atatttgctt atacaatcat cctgtttttg gggcttttct 7561 gattatcaat cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt 7621 ctcttgtttg ctccagactt tcaggtaatg acctgatagc ctttgtagac ctctcaaaaa 7681 tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgacggtg 7741 atttgactgt ctccggcctt tctcacccgt ttgaatcttt gcctactcat tactccggca 7801 ttgcatttaa aatatatgag ggttctaaaa atttttatcc ctgcgttgaa attaaggctt 7861 caccagcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct 7921 ctgaggcttt attgcttaat tttgctaact ctctgccttg cttgtacgat ttattggatg 7981 tt //