LOCUS       pADL-23Chlo             3869 bp    DNA     circular
DEFINITION  Phage display vector pADL-23Chlo, complete sequence.
ACCESSION   
VERSION     .1
KEYWORDS    .
COMMENT     
FEATURES             Location/Qualifiers
     CDS             236..895
                     /codon_start=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /gene="chloramphenicol acetyltransferase"
                     /label=CAT
                     /note
     rep_origin      1050..1669
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_binding    1799..1819
                     /label=O3_operator
     misc_signal     1820..1840
                     /note="CAP binding site"
                     /label=CAP_binding_site
     -35_signal      1855..1860
     -10_signal      1879..1884
     misc_feature    1890
                     /label=start
                     /note="transcription start of the lac operon"
     misc_binding    1891..1911
                     /label=O1_operator
     primer_bind     1899..1928
                     /label=phiS4
     misc_binding    1913..1918
                     /db_xref="REBASE:EcoRI"
     CDS             1934..1996
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     misc_binding    1955..1962
                     /db_xref="REBASE:NotI"
     misc_binding    1981..1993
                     /db_xref="REBASE:SfiI"
     misc_binding    1982..1992
                     /db_xref="REBASE:BglI"
     misc_binding    1996..2001
                     /db_xref="REBASE:SpeI"
     misc_binding    2002..2014
                     /db_xref="REBASE:SfiI"
     misc_binding    2003..2013
                     /db_xref="REBASE:BglI"
     CDS             2077..3297
                     /gene="gene III"
                     /codon_start=1
                     /product="pIII"
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /label=g3p
     primer_bind     complement(2107..2134)
                     /label=psiR2
     primer_bind     complement(2145..2168)
                     /label=psiR3
     misc_binding    2664..2669
                     /db_xref="REBASE:BamHI"
     misc_binding    3298..3303
                     /db_xref="REBASE:NheI"
     terminator      3307..3419
                     /note="lambda t1 terminator"
     rep_origin      3540..3846
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
     CDS             2002..2076
                     /codon_start=1
                     /translation="GPGGQHHHHHHGAEQKLISEEDLS*"
                     /gene
                     /label=Linker
                     /note
     misc_feature    2017..2034
                     /gene
                     /label=His-Tag
                     /note
     misc_feature    2041..2070
                     /gene
                     /label=Myc-Tag
                     /note
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgcgc ttacaatttc aggtggcact
       61 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      121 tatccgctca tgtcgagacg ttgggtgagg ttccaacttt caccataatg aaataagatc
      181 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
      241 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      301 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      361 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      421 tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag acggtgagct
      481 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
      541 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
      601 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
      661 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
      721 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
      781 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
      841 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaactgtc
      901 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
      961 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1201 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1681 gttcctggcc ttttgctggc cttttgctca catgcgccca atacgcaaac cgcctctccc
     1741 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1801 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1861 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttgaattcaa
     1921 ggagacagtc ataatgaaat acctattgcc tacggcggcc gctggattgt tattactcgc
     1981 ggcccagccg gcctaactag tggcccggga ggccaacacc atcaccacca tcatggcgca
     2041 gaacaaaaac tcatctcaga agaggatctg tcttaggccg aaactgttga aagttgttta
     2101 gcaaaacctc atacagaaaa ttcatttact aacgtctgga aagacgacaa aactttagat
     2161 cgttacgcta actatgaggg ctgtctgtgg aatgctacag gcgttgtggt ttgtactggt
     2221 gacgaaactc agtgttacgg tacatgggtt cctattgggc ttgctatccc tgaaaatgag
     2281 ggtggtggct ctgagggtgg cggttctgag ggtggcggtt ctgagggtgg cggtactaaa
     2341 cctcctgagt acggtgatac acctattccg ggctatactt atatcaaccc tctcgacggc
     2401 acttatccgc ctggtactga gcaaaacccc gctaatccta atccttctct tgaggagtct
     2461 cagcctctta atactttcat gtttcagaat aataggttcc gaaataggca gggtgcatta
     2521 actgtttata cgggcactgt tactcaaggc actgaccccg ttaaaactta ttaccagtac
     2581 actcctgtat catcaaaagc catgtatgac gcttactgga acggtaaatt cagagactgc
     2641 gctttccatt ctggctttaa tgaggatcca ttcgtttgtg aatatcaagg ccaatcgtct
     2701 gacctgcctc aacctcctgt caatgctggc ggcggctctg gtggtggttc tggtggcggc
     2761 tctgagggtg gcggctctga gggtggcggt tctgagggtg gcggctctga gggtggcggt
     2821 tccggtggcg gctccggttc cggtgatttt gattatgaaa aaatggcaaa cgctaataag
     2881 ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa aggcaaactt
     2941 gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga cgtttccggc
     3001 cttgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca aatggctcaa
     3061 gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt accttctttg
     3121 cctcagtcgg ttgaatgtcg cccttatgtc tttggcgctg gtaaaccata tgaattttct
     3181 attgattgtg acaaaataaa cttattccgt ggtgtctttg cgtttctttt atatgttgcc
     3241 acctttatgt atgtattttc gacgtttgct aacatactgc gtaataagga gtcttaagct
     3301 agctaacagt cctatgaatc aactacttag atggtattag tgacctgtaa cagagcatta
     3361 gcgcaaggtg atttttgtct tcttgcgcta attttttgtc atcaaacctg tcgcactcct
     3421 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3481 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3541 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3601 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3661 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3721 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3781 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3841 taatgcgccg ctacagggcg cgtcaggtg
//
