LOCUS       pADL-43                 3280 bp    DNA     circular
DEFINITION  Phage display vector pADL-43, complete sequence.
ACCESSION   
VERSION     .0
KEYWORDS    .
COMMENT     
FEATURES             Location/Qualifiers
     promoter        56..84
                     /label=AmpR_Promoter
     CDS             126..986
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     rep_origin      1141..1760
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_binding    1890..1910
                     /label=O3
     misc_signal     1911..1931
                     /label=CAP_binding_site
                     /note="CAP binding site"
     -35_signal      1946..1951
     -10_signal      1970..1975
     misc_feature    1981
                     /label=start
                     /note="transcription start of the lac operon"
     misc_binding    1982..2002
                     /label=operator
     primer_bind     1990..2019
                     /label=phiS4
     misc_binding    2004..2009
                     /db_xref="REBASE:EcoRI"
     CDS             2025..2087
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     misc_binding    2046..2053
                     /db_xref="REBASE:NotI"
     misc_binding    2072..2084
                     /db_xref="REBASE:SfiI"
     misc_binding    2073..2083
                     /db_xref="REBASE:BglI"
     misc_binding    2087..2092
                     /db_xref="REBASE:MluI"
     misc_binding    2093..2098
                     /db_xref="REBASE:SpeI"
     misc_binding    2099..2111
                     /db_xref="REBASE:SfiI"
     misc_binding    2100..2110
                     /db_xref="REBASE:BglI"
     primer_bind     complement(2169..2188)
                     /label=psiR4
     misc_binding    2708..2713
                     /db_xref="REBASE:NheI"
     terminator      2717..2830
                     /label=lt1_terminator
     rep_origin      2951..3257
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
     misc_feature    2114..2131
                     /gene
                     /label=His-Tag
                     /note
     misc_feature    2138..2167
                     /gene
                     /label=Myc-Tag
                     /note
     CDS             2255..2707
                     /codon_start=1
                     /translation="DFDYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAID
                     GFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYV
                     FGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANILRNKES*"
                     /gene="gene III "
                     /label=g3pCT
                     /note="C-Terminal Domain"
     CDS             2099..2254
                     /codon_start=1
                     /translation="GPGGQHHHHHHGAEQKLISEEDLS*EGGGSEGGGSEGGGSEGGG
                     SGGGSGSG"
                     /gene
                     /label=Linker
                     /note
     misc_feature    2174..2254
                     /gene
                     /label=N2-CT_Linker
                     /note
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 gatctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggcctaacgc gtactagtgg
     2101 cccgggaggc caacaccatc accaccatca tggcgcagaa caaaaactca tctcagaaga
     2161 ggatctgtct taggagggtg gcggctctga gggtggcggt tctgagggtg gcggctctga
     2221 gggtggcggt tccggtggcg gctccggttc cggtgatttt gattatgaaa aaatggcaaa
     2281 cgctaataag ggggctatga ccgaaaatgc cgatgaaaac gcgctacagt ctgacgctaa
     2341 aggcaaactt gattctgtcg ctactgatta cggtgctgct atcgatggtt tcattggtga
     2401 cgtttccggc cttgctaatg gtaatggtgc tactggtgat tttgctggct ctaattccca
     2461 aatggctcaa gtcggtgacg gtgataattc acctttaatg aataatttcc gtcaatattt
     2521 accttctttg cctcagtcgg ttgaatgtcg cccttatgtc tttggcgctg gtaaaccata
     2581 tgaattttct attgattgtg acaaaataaa cttattccgt ggtgtctttg cgtttctttt
     2641 atatgttgcc acctttatgt atgtattttc gacgtttgct aacatactgc gtaataagga
     2701 gtcttaagct agctaacagt cactatgaat caactactta gatggtatta gtgacctgta
     2761 acagagcatt agcgcaaggt gatttttgtc ttcttgcgct aattttttgt catcaaacct
     2821 gtcgcactcc ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2881 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     2941 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3001 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3061 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3121 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3181 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3241 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
//
