Custom Immunized Phage Display Antibody Libraries

Service Description

Phage display is a straightforward approach to isolate high affinity monoclonal antibodies from small, focused libraries build from immunized animals. These libraries are of moderate size, between 1 and 10 million clones and usually mirror the animal immune response; high affinity antibodies, similar to antibody generated by hybridoma technology, are isolated from them by straightforward selection techniques. We have extensive experience in building phage antibody libraries in varied formats and display types. You provide the antigen; we conduct the immunization, design and build the antibody library for you as a service.

Murine antibody libraries.

Phage display enables very selective screens to isolate clones that are not always accessible by classical hybridoma technology. We will immunize five mice with your antigen; the spleen of the best responder will be harvested and used to amplify cDNA and build the library. We can provide you with the bleeds to make your own testing.

Custom Rabbit Monoclonal Antibodies by Phage Display

Rabbits are a great source of antibodies; there are outbred animals mounting very strong immunue responses to most antigens, including many antigens that do not elicit a significant response in inbred laboratory mice and small haptens in particular and constitute a good alternative to mice when monoclonal antibodies are needed. We will immunize two rabbits with your antigen; the spleen of the best responder will be harvested and used to amplify cDNA and build the library. We can provide you with the bleeds to make your own testing.

Antibody Format & Display Type

You can choose from any of our phagemid vectors to build the library or you can provide your own vector. We can build scFv and Fab libraries; specific format and tags will be decided together.

Library Size

The size of the library will be tailored to your project. Our standard size is 1 x 10(8) guaranteed in-frame antibody clones.

Cost & Turnaround time

P

lease, request a specific quote by completing the online quotation form accessible by pressing this button; you will be contacted back by the next business day.

Request A Quote

For all other inquiries, contact us by email at info@abdesignlabs.com or by phone at 1-877-223-3104 (PST). We guarantee competitive pricing.

All our work is performed by highly experienced staff at our San Diego facility in California. We use highly efficient cloning techniques to ensure timely delivery and have very high standards for validation. All products and services are for research use only and not intended for use in humans.

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