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TGEX-FH

MX004


Price: $455.00

TEGX-FH

Heavy Chain Cloning Vector for the Expression of Fab Fragments

Technical Description

The TGEX vector series is designed for the rapid expression of antibody molecules by transient gene expression in mammalian cell suspension culture. TGEX™-FH expresses the CH1 constant region of the human IgG1 heavy chain. Expression of Fab fragments is obtained by co-transfection with a light chain variable region cloned into the vector TGEX™-LC. Using transient transfection technologies yields of Fab fragment between 10 mg/L and 100 mg/L in serum-free conditions can be obtained in just a few days.

Applications

  • Mammalian expression of Fab fragments by transient gene expression.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Plasmid Size: 3668
Promoter: CMV + TPL
Leader Peptide: IGHV1-2
Cloning Site: BssHII + BsmBI
Fusion Protein: human IgG1 CH1 constant region
Selection: ampicillin
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Product Sequence:

The entire plasmid sequence was confirmed by the Sanger method.

Certification:

Product meets all specifications.