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TGEX-HChis

MX005


Price: $455.00

TEGX-HChis

Heavy Chain Cloning Vector for the Expression of Full Length Antibodies

Technical Description

The TGEX vector series is designed for the rapid expression of antibody molecules by transient gene expression in mammalian cell suspension culture. TGEX™-HChis expresses the constant region of the human IgG1 heavy chain with a C-terminal HIS tag for IMAC purificaiton. Expression of full length antibody molecules is achieved by co-transfection with a light chain variable region cloned into the vector TGEX™-LC. Using transient transfection technologies yields of antibody between 10 mg/L and 100 mg/L in serum-free conditions can be obtained in just a few days.

Applications

  • Mammalian expression of full length antibodies by transient gene expression.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Plasmid Size: 4343
Promoter: CMV + TPL
Leader Peptide: IGHV1-2
Cloning Site: BssHII + BsmBI
Fusion Protein: full length IgG1 human constant region
Selection: ampicillin
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Product Sequence:

The entire plasmid sequence was confirmed by the Sanger method.

Certification:

Product meets all specifications.