Price (USD): $498.00


Cloning Vector for the Expression of scFv-Fc Fusion Molecules for Phage Display

Technical Description

The TGEX™-SCblue vector is designed for the mammalian expression of scFv-Fc fusions after transfer of scFv fragments from the PADL™ phagemid vector series.  TGEX™-SCblue vector enables rapid and convenient expression of scFv fragment isolated by phage display as dimeric scFv-Fc fusion with all the advantages conferred by the Fc fragment for detection using commercially available secondary antibodies. Transfer of the scFv inserts are done seamlessly through a common double-SfiI (Fig. 1). Proper recombinant clones can be isolated using a blue/white colony screening.


Figure 1. scFv Phage Display to scFv-Fc Expression. Expression of free scFv from multiple phage clones is not an easy task in bacteria and binding is often weak because of the monovalency of scFv. Using TGEX-SCblue scFv fragments can be easily transferred to a convenient mammalian transient expression system as scFv-Fc fusion (Valadon (2006), Yoon (2012)). With yields in the 10 to 100 mg/L range, just a few ml of culture is enough to validate your antibodies in any assay using simple secondary antibodies.


  • Mammalian expression of scFv-Fc or VHH-Fc fusion molecules in combination with phage display

For research use only; not intended for any animal or human therapeutic or diagnostic use.


General Characteristics:

Plasmid Size: 4323
Promoter: CMV + TPL
Leader Peptide: pelBK
Cloning Site: Double SfiI site compatible with pADL phagemid vectors
Fusion Protein: human IgG1 Fc constant region
Selection: ampicillin
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Product Sequence:

The entire plasmid sequence was confirmed by the Sanger method.


Product meets all specifications.


  1. Valadon, P., Garnett, J.D., Testa, J.E., Bauerle, M., Oh, P. and Schnitzer, J.E. (2006). Proc Natl Acad Sci U S A. 103(2):407-12.
  2. Yoon, H., Song, J. M., Ryu, C. J., Kim, Y.-G., Lee, E. K., Kang, S., & Kim, S. J. (2012). BMC Biotechnology, 12(1), 62.