Antibody Expression with the TGEX Vector Series

Antibody Expression with the TGEX Vector Series

A tool for rapid transient expression of antibodies.

P

hage display only produces phage clones. Between them and fully recombinant antibodies expressed in stable cell lines lies a grey zone where rapid access to free, recombinant molecules becomes a necessity. The TGEX™ transient expression vector series has been designed to bridge this gap and allows for the rapid expression of recombinant antibodies by transient transfection.

The TGEX Vector Backbone


The TGEX™-Zeo backbone features a CMV promoter, the adenovirus tripartite leader sequence (TPL) (Logan 1984, Mariati 2010), a powerful hybrid leader sequence, a convenient cloning site to insert the antibody sequence, followed by an IRES element and the Sh ble gene from Streptoalloteichus hindustanus conferring resistance to Zeocin and terminated by the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and the rabbit beta-globin polyadenylation signal (BGPA). Using widely available cell lines and large scale transfection technologies, yields of antibody between 20 mg/L and 200 mg/L, or more, are routinely achieved in the laboratory in a few days. Selection with Zeocin results in the creation of stable pools with, in the case of two plasmids, an equal distribution of the expressions, here illustrated with eGFP and DsRed, allowing the concomitant selection of the antibody heavy chain and the antibody light chain with a single antibiotic.

Large Collection of Isotypes Available

TGEX Vector

Cat#

Isotype

Human Antibodies

TGEX-HC-hG1-Zeo

MX026

Human IgG1 heavy chain expression vector

TGEX-HC-hG2-Zeo

MX027

Human IgG2 heavy chain expression vector

TGEX-HC-hG3-Zeo

MX028

Human IgG3 heavy chain expression vector

TGEX-HC-hG4[S228P]-Zeo

MX029

Human IgG4 heavy chain expression vector

TGEX-LC-hK-Zeo

MX030

Human Kappa light chain expression vector

TGEX-LC-hL2-Zeo

MX031

Human Lambda 2 light chain expression vector

Fc-Engineered Antibodies

With Enhanced Fc-Effector Function

TGEX-HC-hG1[EA]-Zeo

MX041

Human IgG1 heavy chain with increased ADCC and CDC in vitro

TGEX-HC-hG1[NA]-Zeo

MX042

Human IgG1 aglycosylated heavy chain

TGEX-HC-hG1[LALA-PG]-Zeo

MX043

Human IgG1 heavy chain with decreased ADCC and CDC in vitro

TGEX-HC-hG1[YTE-KF]-Zeo

MX044

Human IgG1 heavy chain with increased antibody serum half-life

TGEX-HC-hG4[SPLE-PG]-Zeo

MX045

Human IgG4 heavy chain with decreased ADCC and CDC in vitro

Murine Antibodies

TGEX-HC-mG1-Zeo

MX032

Murine IgG1 heavy chain expression vector

TGEX-HC-mG2a-Zeo

MX033

Murine IgG2a heavy chain expression vector

TGEX-HC-mG2b-Zeo

MX034

Murine IgG2b heavy chain expression vector

TGEX-HC-mG3-Zeo

MX035

Murine IgG3 heavy chain expression vector

TGEX-LC-mK-Zeo

MX036

Murine Kappa light chain expression vector

TGEX-HC-mL1-Zeo

MX037

Murine Lambda 1 light chain expression vector

TGEX-HC-mL2-Zeo

MX038

Murine Lambda 2 light chain expression vector

Rabbit Antibodies

TGEX-HC-rbG-Zeo

MX039

Rabbit IgG heavy chain expression vector

TGEX-LC-rbKb4-Zeo

MX040

Rabbit Kappa light chain expression vector

Dog Antibodies

TGEX-HC-dG1-Zeo

MX046

Dog IgG1 heavy chain expression vector

TGEX-HC-dG2-Zeo

MX047

Dog IgG2 heavy chain expression vector

TGEX-HC-dG3-Zeo

MX048

Dog IgG3 heavy chain expression vector

TGEX-HC-dG4-Zeo

MX049

Dog IgG4 heavy chain expression vector

TGEX-LC-dK-Zeo

MX050

Dog Kappa light chain expression vector

TGEX-LC-dL-Zeo

MX051

Dog Lambda light chain expression vector

Other Species

  Please, inquire!

Adapted to Multiple Antibody Formats

Fab fragments, Fc fusions, scFv-Fc/VHH fusion, secreted proteins.

TGEX Vector

Cat#

Isotype

TGEX-AC-Zeo

MX020

Generic vector for the secretion of recombinant proteins

TGEX-FC-hG1-Zeo

MX025

Expression of human IgG1 Fc fusions

TGEX-FH-hG1-Zeo

MX023

Expression of human or chimeric IgG1 Fab fragment

TGEX-SCblue-Zeo

MX024

Conversion of scFv or VHH on phage into Fc fusions

Control Vectors

TGEX Vector

Cat#

Isotype

TGEX-eGFP-Zeo

MX022

Green fluorescent reporter vector

Vector maps, sequences, full description and ordering…

The TGEX-SCblue vector is designed for the conversion of scFv-bearing phage clones into scFv-Fc fusions for antibody validation after biopanning.

References

  • Logan, J., & Shenk, T. (1984). Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection. Proceedings of the National Academy of Sciences of the United States of America, 81(12), 3655–9.

  • Mariati, Ho, S. C. L., Yap, M. G. S., & Yang, Y. (2010). Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells. Protein Expression and Purification, 69(1), 9–15.

PUB010 – Rev221230

Please send comments to info@abdesignlabs.com.

Copyright © 2022 Antibody Design Labs. All rights reserved. The reuse or reproduction of any of the information, design or layout contained in this web site without the permission of Antibody Design Labs is prohibited.

Comments are closed.