LOCUS       M13KO7                  8668 bp ss-DNA     circular     10-OCT-2015
DEFINITION  M13KO7 Helper Phage.
ACCESSION   
VERSION     .2
KEYWORDS    .
COMMENT     Sequenced by Antibody Design Labs.
FEATURES             Location/Qualifiers
     gene            join(8267..8668,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /db_xref="GeneID:927328"
     CDS             join(8267..8668,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /note="The pII protein binds to the intergenic region in
                     double-stranded, supercoiled DNA (replicative form) and
                     nicks the positive strand.  The resulting free 3' OH
                     terminus serves as a primer for new strand synthesis using
                     host factors (Rep helicase, DNA polymerase III). After a
                     full round of replication, pII circularizes the displaced
                     DNA strand. Newly synthesized (+) single stranded DNA is
                     either converted to RF DNA or packaged as genomes,
                     depending on the concentrations of ssDNA binding protein pV
                     and a separately expressed fragment of pII termed pX.
                     Functions: DNA replication initiation"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="NP_510885.1"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA
                     RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA*"
                     /db_xref="GI:17426218"
                     /db_xref="GeneID:927328"
     gene            496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /db_xref="GeneID:927329"
     CDS             496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /note="Protein pX is synthesized from an in-frame internal
                     initiation codon in gene II and is identical to the
                     C-terminal third of pII.  Required for the accumulation of
                     (+) single stranded genomic DNA."
                     /codon_start=1
                     /transl_table=11
                     /product="gene X product"
                     /protein_id="NP_510886.1"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
                     /db_xref="GI:17426219"
                     /db_xref="GeneID:927329"
     gene            843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /db_xref="GeneID:927330"
     CDS             843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /codon_start=1
                     /transl_table=11
                     /product="helix destabilising protein"
                     /protein_id="NP_510887.1"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
                     /db_xref="GI:17426220"
                     /db_xref="GeneID:927330"
     old_sequence    898
                     /gene="V"
                     /locus_tag="M13p03"
                     /citation=[1]
                     /replace="g"
     gene            1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /db_xref="GeneID:927331"
     CDS             1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510888.1"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
                     /db_xref="GI:17426221"
                     /db_xref="GeneID:927331"
     gene            1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /db_xref="GeneID:927332"
     CDS             1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510889.1"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
                     /db_xref="GI:17426222"
                     /db_xref="GeneID:927332"
     gene            1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /db_xref="GeneID:927333"
     CDS             1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /note="Several thousand copies of the major coat protein
                     form the tube containing the ssDNA genome to form the
                     elongated body of filamentous phages."
                     /codon_start=1
                     /transl_table=11
                     /product="major coat protein"
                     /protein_id="NP_510890.1"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY
                     IGYAWAMVVVIVGATIGIKLFKKFTSKAS*"
                     /db_xref="GI:17426223"
                     /db_xref="GeneID:927333"
     gene            1579..2853
                     /gene="III"
                     /locus_tag="M13p07"
                     /db_xref="GeneID:927334"
     CDS             1579..2853
                     /gene="III"
                     /locus_tag="M13p07"
                     /note="Minor coat protein pIII is located at the tail of
                     the filamentous phage particle.  It is involved in
                     termination of assembly and infectivity. In the absence of
                     pIII abnormally long phage particles are formed and remain
                     attached to host cells. In addition, pIII is involved in
                     initiating infection of host cells by binding to pili. In
                     the case of the Ff phages, pIII binds to the tip of F-pili
                     and then interacts with the TolA protein. The assembly
                     termination function maps to the C-terminus of pIII,
                     whereas the receptor binding maps to the N-terminus. Ike
                     and f1 proteins cannot replace one another. Functions:
                     receptor binding, morphogenesis, structural."
                     /codon_start=1
                     /transl_table=11
                     /product="attachment protein"
                     /protein_id="NP_510891.1"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKT
                     LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG
                     GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR
                     NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFV
                     CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF
                     DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT
                     GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFSAGKPYEFSIDCDKI
                     NLFRGVFAFLLYVATFMYVFSTFANILRNKES"
                     /db_xref="GI:17426224"
                     /db_xref="GeneID:927334"
     gene            2856..3194
                     /gene="VI"
                     /locus_tag="M13p08"
                     /db_xref="GeneID:927335"
     CDS             2856..3194
                     /gene="VI"
                     /locus_tag="M13p08"
                     /note="The pVI proteins of Ff phages are minor coat
                     proteins that are located at the end of the virion.  They
                     are involved in termination of assembly. In the absence of
                     pVI, phage particles remain associated with the host
                     membrane."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510892.1"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK"
                     /db_xref="GI:17426225"
                     /db_xref="GeneID:927335"
     gene            3196..4242
                     /gene="I"
                     /locus_tag="M13p09"
                     /db_xref="GeneID:927336"
     CDS             3196..4242
                     /gene="I"
                     /locus_tag="M13p09"
                     /note="The pI protein of filamentous phages is involved in
                     morphogenesis and may hydrolyze ATP. It is an inner
                     membrane protein and interacts with the outer membrane
                     protein pIV.  The N-termini of members of this cluster
                     share homology to the zonular occludens toxin (Zot) encoded
                     by phage CTXphi. However, the toxin activity of Zot was
                     mapped to the C terminus, which is divergent in the
                     proteins in this cluster. pXI is the product of
                     translational reinitiationin gene I and interacts with pI."
                     /codon_start=1
                     /transl_table=11
                     /product="assembly protein"
                     /protein_id="NP_510893.1"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV
                     SIKKGNSNEIVKCN"
                     /db_xref="GI:17426226"
                     /db_xref="GeneID:927336"
     gene            4220..5500
                     /gene="IV"
                     /locus_tag="M13p10"
                     /db_xref="GeneID:927337"
     CDS             4220..5500
                     /gene="IV"
                     /locus_tag="M13p10"
                     /note="pIV proteins of filamentous phages are multimeric
                     proteins that form a channel in the outer membrane through
                     which the phage particles exit the host cell. Function:
                     morphogenesis."
                     /codon_start=1
                     /transl_table=11
                     /product="phage assembly protein"
                     /protein_id="NP_510894.1"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNNQDYI
                     DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL"
                     /db_xref="GI:17426227"
                     /db_xref="GeneID:927337"
     rep_origin      5488..5830
                     /note="M13 origin of replication (- +) (fragment)"
     rep_origin      complement(6046..6591)
                     /note="p15A origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     gene            complement(7140..7955)
                     /gene="aph(3')-Ia"
     CDS             complement(7140..7955)
                     /gene="aph(3')-Ia"
                     /note="kanR (confers resistance to kanamycin)"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      8092..8129
                     /note="M13 origin of replication (- +) (fragment)"
     misc_binding    5825..5830
                     /db_xref="REBASE:AvaI"
     misc_binding    2220..2225
                     /db_xref="REBASE:BamHI"
     misc_binding    7401..7406
                     /db_xref="REBASE:HindIII"
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagccct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     1621 tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     1681 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     1741 ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     1801 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     1861 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     1921 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     1981 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     2041 cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     2101 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     2161 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     2221 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     2281 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     2341 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     2401 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     2461 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     2521 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     2581 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     2641 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     2701 tttgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     2761 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     2821 tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     2881 tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     2941 ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     3001 ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     3061 ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     3121 tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     3181 attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     3241 ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     3301 cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     3361 cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     3421 tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     3481 acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     3541 aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     3601 cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     3661 tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     3721 gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     3781 actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     3841 tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     3901 aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     3961 tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     4021 gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     4081 cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     4141 agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     4201 attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     4261 tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     4321 tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     4381 tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     4441 tgttttacgt gcaaataatt ttgatatggt aggttctaac ccttccatta ttcagaagta
     4501 taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     4561 tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     4621 ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     4681 gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     4741 tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc
     4801 aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     4861 tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     4921 cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     4981 agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     5041 tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     5101 tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     5161 tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     5221 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     5281 tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     5341 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
     5401 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
     5461 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     5521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5701 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5761 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5821 ctatctcggg acggatcgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc
     5881 agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
     5941 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
     6001 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
     6061 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     6121 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
     6181 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
     6241 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     6301 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg
     6361 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca
     6421 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag
     6481 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg
     6541 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat
     6601 cttattaagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccgattc gagctcgccc
     6961 ggggatcgac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     7021 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg
     7081 ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat
     7141 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     7201 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     7261 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     7321 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     7381 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     7441 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     7501 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     7561 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     7621 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     7681 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     7741 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     7801 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     7861 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     7921 ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     7981 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     8041 agattttgag acacaacgtg gctttccccc cccccccctg caggtctcgg gctattcttt
     8101 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8161 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac
     8221 aatcttcctg tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct
     8281 agttttacga ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct
     8341 gatagccttt gtagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag
     8401 aacggttgaa tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga
     8461 atctttacct acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt
     8521 ttatccttgc gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt
     8581 tggtacaacc gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt
     8641 gccttgcctg tatgatttat tggatgtt
//

