LOCUS       M13KO7d3                7466 bp ss-DNA     circular     07-OCT-2015
DEFINITION  M13KO7d3 pIII-Defective Helper Phage.
ACCESSION   
VERSION     .2
KEYWORDS    .
COMMENT     FT color
            FT join(1579..1632,1633..1651)
            FT /number="12"
            FT translated
            FT join(1633..1638,1639..1641)
            FT /frame="1"
            FT translated
            FT 1633
            FT /frame="2"
            FT translated
            FT 1633..1634
            FT /frame="3"
FEATURES             Location/Qualifiers
     gene            join(7065..7466,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /db_xref="GeneID:927328"
     CDS             join(7065..7466,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /note="The pII protein binds to the intergenic region in
                     double-stranded, supercoiled DNA (replicative form) and
                     nicks the positive strand.  The resulting free 3' OH
                     terminus serves as a primer for new strand synthesis using
                     host factors (Rep helicase, DNA polymerase III). After a
                     full round of replication, pII circularizes the displaced
                     DNA strand. Newly synthesized (+) single stranded DNA is
                     either converted to RF DNA or packaged as genomes,
                     depending on the concentrations of ssDNA binding protein pV
                     and a separately expressed fragment of pII termed pX.
                     Functions: DNA replication initiation"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="NP_510885.1"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA
                     RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA*"
                     /db_xref="GI:17426218"
                     /db_xref="GeneID:927328"
     gene            496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /db_xref="GeneID:927329"
     CDS             496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /note="Protein pX is synthesized from an in-frame internal
                     initiation codon in gene II and is identical to the
                     C-terminal third of pII.  Required for the accumulation of
                     (+) single stranded genomic DNA."
                     /codon_start=1
                     /transl_table=11
                     /product="gene X product"
                     /protein_id="NP_510886.1"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
                     /db_xref="GI:17426219"
                     /db_xref="GeneID:927329"
     gene            843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /db_xref="GeneID:927330"
     CDS             843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /codon_start=1
                     /transl_table=11
                     /product="helix destabilising protein"
                     /protein_id="NP_510887.1"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
                     /db_xref="GI:17426220"
                     /db_xref="GeneID:927330"
     old_sequence    898
                     /gene="V"
                     /locus_tag="M13p03"
                     /citation=[1]
                     /replace="g"
     gene            1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /db_xref="GeneID:927331"
     CDS             1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510888.1"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
                     /db_xref="GI:17426221"
                     /db_xref="GeneID:927331"
     gene            1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /db_xref="GeneID:927332"
     CDS             1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510889.1"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
                     /db_xref="GI:17426222"
                     /db_xref="GeneID:927332"
     gene            1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /db_xref="GeneID:927333"
     CDS             1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /note="Several thousand copies of the major coat protein
                     form the tube containing the ssDNA genome to form the
                     elongated body of filamentous phages."
                     /codon_start=1
                     /transl_table=11
                     /product="major coat protein"
                     /protein_id="NP_510890.1"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY
                     IGYAWAMVVVIVGATIGIKLFKKFTSKAS*"
                     /db_xref="GI:17426223"
                     /db_xref="GeneID:927333"
     CDS             1579..>1632
                     /gene="III"
                     /locus_tag="M13p07"
                     /note="Minor coat protein pIII is located at the tail of
                     the filamentous phage particle.  It is involved in
                     termination of assembly and infectivity. In the absence of
                     pIII abnormally long phage particles are formed and remain
                     attached to host cells. In addition, pIII is involved in
                     initiating infection of host cells by binding to pili. In
                     the case of the Ff phages, pIII binds to the tip of F-pili
                     and then interacts with the TolA protein. The assembly
                     termination function maps to the C-terminus of pIII,
                     whereas the receptor binding maps to the N-terminus. Ike
                     and f1 proteins cannot replace one another. Functions:
                     receptor binding, morphogenesis, structural."
                     /codon_start=1
                     /transl_table=11
                     /product="attachment protein"
                     /protein_id="NP_510891.1"
                     /translation="VKKLLFAIPLVVPFYSHS"
                     /db_xref="GI:17426224"
                     /db_xref="GeneID:927334"
     gene            1654..1992
                     /gene="VI"
                     /locus_tag="M13p08"
                     /db_xref="GeneID:927335"
     CDS             1654..1992
                     /gene="VI"
                     /locus_tag="M13p08"
                     /note="The pVI proteins of Ff phages are minor coat
                     proteins that are located at the end of the virion.  They
                     are involved in termination of assembly. In the absence of
                     pVI, phage particles remain associated with the host
                     membrane."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510892.1"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK*"
                     /db_xref="GI:17426225"
                     /db_xref="GeneID:927335"
     gene            1994..3040
                     /gene="I"
                     /locus_tag="M13p09"
                     /db_xref="GeneID:927336"
     CDS             1994..3040
                     /gene="I"
                     /locus_tag="M13p09"
                     /note="The pI protein of filamentous phages is involved in
                     morphogenesis and may hydrolyze ATP. It is an inner
                     membrane protein and interacts with the outer membrane
                     protein pIV.  The N-termini of members of this cluster
                     share homology to the zonular occludens toxin (Zot) encoded
                     by phage CTXphi. However, the toxin activity of Zot was
                     mapped to the C terminus, which is divergent in the
                     proteins in this cluster. pXI is the product of
                     translational reinitiationin gene I and interacts with pI."
                     /codon_start=1
                     /transl_table=11
                     /product="assembly protein"
                     /protein_id="NP_510893.1"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV
                     SIKKGNSNEIVKCN*"
                     /db_xref="GI:17426226"
                     /db_xref="GeneID:927336"
     gene            3018..4298
                     /gene="IV"
                     /locus_tag="M13p10"
                     /db_xref="GeneID:927337"
     CDS             3018..4298
                     /gene="IV"
                     /locus_tag="M13p10"
                     /note="pIV proteins of filamentous phages are multimeric
                     proteins that form a channel in the outer membrane through
                     which the phage particles exit the host cell. Function:
                     morphogenesis."
                     /codon_start=1
                     /transl_table=11
                     /product="phage assembly protein"
                     /protein_id="NP_510894.1"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYI
                     DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*"
                     /db_xref="GI:17426227"
                     /db_xref="GeneID:927337"
     rep_origin      4286..4628
                     /note="M13 origin of replication (- +) (fragment)"
     rep_origin      complement(4844..5389)
                     /note="p15A origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     gene            complement(5938..6753)
                     /gene="aph(3')-Ia"
     CDS             complement(5938..6753)
                     /gene="aph(3')-Ia"
                     /note="kanR (confers resistance to kanamycin)"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      6890..6927
                     /note="M13 origin of replication (- +) (fragment)"
     misc_binding    4623..4628
                     /db_xref="REBASE:AvaI"
     misc_binding    6199..6204
                     /db_xref="REBASE:HindIII"
     primer_bind     1486..1510
                     /label=m13g8_s
     primer_bind     complement(4386..4410)
                     /label=fd5589_r
     primer_bind     complement(3357..3378)
                     /label=m13g4_r
     primer_bind     904..926
                     /label=m13g5_s
     primer_bind     4030..4054
                     /label=m13g4c_s
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagccct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     1621 tattctcact ccgcgtaata aggagtctta atcatgccag ttcttttggg tattccgtta
     1681 ttattgcgtt tcctcggttt ccttctggta actttgttcg gctatctgct tacttttctt
     1741 aaaaagggct tcggtaagat agctattgct atttcattgt ttcttgctct tattattggg
     1801 cttaactcaa ttcttgtggg ttatctctct gatattagcg ctcaattacc ctctgacttt
     1861 gttcagggtg ttcagttaat tctcccgtct aatgcgcttc cctgttttta tgttattctc
     1921 tctgtaaagg ctgctatttt catttttgac gttaaacaaa aaatcgtttc ttatttggat
     1981 tgggataaat aatatggctg tttattttgt aactggcaaa ttaggctctg gaaagacgct
     2041 cgttagcgtt ggtaagattc aggataaaat tgtagctggg tgcaaaatag caactaatct
     2101 tgatttaagg cttcaaaacc tcccgcaagt cgggaggttc gctaaaacgc ctcgcgttct
     2161 tagaataccg gataagcctt ctatatctga tttgcttgct attgggcgcg gtaatgattc
     2221 ctacgatgaa aataaaaacg gcttgcttgt tctcgatgag tgcggtactt ggtttaatac
     2281 ccgttcttgg aatgataagg aaagacagcc gattattgat tggtttctac atgctcgtaa
     2341 attaggatgg gatattattt ttcttgttca ggacttatct attgttgata aacaggcgcg
     2401 ttctgcatta gctgaacatg ttgtttattg tcgtcgtctg gacagaatta ctttaccttt
     2461 tgtcggtact ttatattctc ttattactgg ctcgaaaatg cctctgccta aattacatgt
     2521 tggcgttgtt aaatatggcg attctcaatt aagccctact gttgagcgtt ggctttatac
     2581 tggtaagaat ttgtataacg catatgatac taaacaggct ttttctagta attatgattc
     2641 cggtgtttat tcttatttaa cgccttattt atcacacggt cggtatttca aaccattaaa
     2701 tttaggtcag aagatgaaat taactaaaat atatttgaaa aagttttctc gcgttctttg
     2761 tcttgcgatt ggatttgcat cagcatttac atatagttat ataacccaac ctaagccgga
     2821 ggttaaaaag gtagtctctc agacctatga ttttgataaa ttcactattg actcttctca
     2881 gcgtcttaat ctaagctatc gctatgtttt caaggattct aagggaaaat taattaatag
     2941 cgacgattta cagaagcaag gttattcact cacatatatt gatttatgta ctgtttccat
     3001 taaaaaaggt aattcaaatg aaattgttaa atgtaattaa ttttgttttc ttgatgtttg
     3061 tttcatcatc ttcttttgct caggtaattg aaatgaataa ttcgcctctg cgcgattttg
     3121 taacttggta ttcaaagcaa tcaggcgaat ccgttattgt ttctcccgat gtaaaaggta
     3181 ctgttactgt atattcatct gacgttaaac ctgaaaatct acgcaatttc tttatttctg
     3241 ttttacgtgc aaataatttt gatatggtag gttctaaccc ttccattatt cagaagtata
     3301 atccaaacaa tcaggattat attgatgaat tgccatcatc tgataatcag gaatatgatg
     3361 ataattccgc tccttctggt ggtttctttg ttccgcaaaa tgataatgtt actcaaactt
     3421 ttaaaattaa taacgttcgg gcaaaggatt taatacgagt tgtcgaattg tttgtaaagt
     3481 ctaatacttc taaatcctca aatgtattat ctattgacgg ctctaatcta ttagttgtta
     3541 gtgctcctaa agatatttta gataaccttc ctcaattcct ttcaactgtt gatttgccaa
     3601 ctgaccagat attgattgag ggtttgatat ttgaggttca gcaaggtgat gctttagatt
     3661 tttcatttgc tgctggctct cagcgtggca ctgttgcagg cggtgttaat actgaccgcc
     3721 tcacctctgt tttatcttct gctggtggtt cgttcggtat ttttaatggc gatgttttag
     3781 ggctatcagt tcgcgcatta aagactaata gccattcaaa aatattgtct gtgccacgta
     3841 ttcttacgct ttcaggtcag aagggttcta tctctgttgg ccagaatgtc ccttttatta
     3901 ctggtcgtgt gactggtgaa tctgccaatg taaataatcc atttcagacg attgagcgtc
     3961 aaaatgtagg tatttccatg agcgtttttc ctgttgcaat ggctggcggt aatattgttc
     4021 tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta
     4081 ctaatcaaag aagtattgct acaacggtta atttgcgtga tggacagact cttttactcg
     4141 gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa
     4201 tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat
     4261 acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4321 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4381 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4441 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4501 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4561 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4621 atctcgggac ggatcgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag
     4681 aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     4741 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     4801 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     4861 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     4921 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     4981 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     5041 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     5101 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     5161 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     5221 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     5281 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     5341 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     5401 tattaagggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5461 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5521 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5581 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5641 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5701 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccgattcga gctcgcccgg
     5761 ggatcgacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg
     5821 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc
     5881 gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta
     5941 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     6001 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     6061 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     6121 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     6181 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc
     6241 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     6301 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     6361 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     6421 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     6481 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     6541 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     6601 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     6661 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     6721 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     6781 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
     6841 attttgagac acaacgtggc tttccccccc cccccctgca ggtctcgggc tattcttttg
     6901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6961 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaatattt gcttatacaa
     7021 tcttcctgtt tttggggctt ttctgattat caaccggggt acatatgatt gacatgctag
     7081 ttttacgatt accgttcatc gattctcttg tttgctccag actctcaggc aatgacctga
     7141 tagcctttgt agacctctca aaaatagcta ccctctccgg catgaattta tcagctagaa
     7201 cggttgaata tcatattgat ggtgatttga ctgtctccgg cctttctcac ccttttgaat
     7261 ctttacctac acattactca ggcattgcat ttaaaatata tgagggttct aaaaattttt
     7321 atccttgcgt tgaaataaag gcttctcccg caaaagtatt acagggtcat aatgtttttg
     7381 gtacaaccga tttagcttta tgctctgagg ctttattgct taattttgct aattctttgc
     7441 cttgcctgta tgatttattg gatgtt
//

