LOCUS       TGEX-HC-mG2b-Zeo        5846 bp    DNA     circular
DEFINITION  Murine BALB/c IgG2b Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3154..3742,3758..3856)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,5606..5634)
            FT /number="1"
            FT color
            FT 2771..3145
            FT /number="20"
            FT color
            FT join(1041..1099,1132..2141)
            FT /number="14"
            FT translated
            FT 2771..3145
            FT /frame="2"
            FT color
            FT 3930..4549
            FT /number="2"
            FT color
            FT join(2164..2174,2177..2676,2678,2681..2737)
            FT /number="7"
            FT color
            FT join(4704..4844,4846..5564)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2135
                     /codon_start=1
                     /translation="AKTTPPSVYPLAPGCGDTTGSSVTSGCLVKGYFPEPVTVTWNSG
                     SLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGP
                     ISTINPCPPCKECHKCPAPNLEGGPSVFIFPPNIKDVLMISLTPKVTCVVVDVSEDDP
                     DVQISWFVNNVEVHTAQTQTHREDYNSTIRVVSTLPIQHQDWMSGKEFKCKVNNKDLP
                     SPIERTISKIKGLVRAPQVYTLPPPAEQLSRKDVSLTCLVVGFNPGDISVEWTSNGHT
                     EENYKDTAPVLDSDGSYFIYSKLNMKTSKWEKTDSFSCNVRHEGLKNYYLKKTISRSP
                     GK"
                     /gene
                     /label=mG2b
                     /note
     misc_feature    2136..2141
                     /gene
                     /label=Stop
                     /note
     misc_binding    2142..2147
                     /db_xref="REBASE:NheI"
     misc_feature    2164..2737
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2210..2229)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2674..2680
                     /label=A7
                     /note
     misc_binding    2759..2764
                     /db_xref="REBASE:XbaI"
     misc_binding    2765..2770
                     /db_xref="REBASE:SalI"
     CDS             2771..3145
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3146..3153
                     /db_xref="REBASE:NotI"
     misc_feature    3154..3742
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3758..3856
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3873..3878
                     /db_xref="REBASE:BglII"
     misc_binding    3879..3884
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3930..4549)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4386)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4704..5564)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5606..5634)
                     /label=AmpR_Promoter
     misc_binding    5697..5702
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagct aaaacaacac
     1141 ccccatcagt ctatccactg gcccctgggt gtggagatac aactggttcc tccgtgacct
     1201 ctgggtgcct ggtcaagggg tacttccctg agccagtgac tgtgacttgg aactctggat
     1261 ccctgtccag cagtgtgcac accttcccag ctctcctgca gtctggactc tacactatga
     1321 gcagctcagt gactgtcccc tccagcacct ggccaagtca gaccgtcacc tgcagcgttg
     1381 ctcacccagc cagcagcacc acggtggaca aaaaacttga gcccagcggg cccatttcaa
     1441 caatcaaccc ctgtcctcca tgcaaggagt gtcacaaatg cccagctcct aacctcgagg
     1501 gtggaccatc cgtcttcatc ttccctccaa atatcaagga tgtactcatg atctccctga
     1561 cacccaaggt cacgtgtgtg gtggtggatg tgagcgagga tgacccagac gtccagatca
     1621 gctggtttgt gaacaacgtg gaagtacaca cagctcagac acaaacccat agagaggatt
     1681 acaacagtac tatccgggtg gtcagcaccc tccccatcca gcaccaggac tggatgagtg
     1741 gcaaggagtt caaatgcaag gtgaacaaca aagacctccc atcacccatc gagagaacca
     1801 tctcaaaaat taaagggcta gtcagagctc cacaagtata cactttgccg ccaccagcag
     1861 agcagttgtc caggaaagat gtcagtctca cttgcctggt cgtgggcttc aaccctggag
     1921 acatcagtgt ggagtggacc agcaatgggc atacagagga gaactacaag gacaccgcac
     1981 cagttcttga ctctgacggt tcttacttca tatatagcaa gctcaatatg aaaacaagca
     2041 agtgggagaa aacagattcc ttctcatgca acgtgagaca cgagggtctg aaaaattact
     2101 acctgaagaa gaccatctcc cggtcaccgg gtaaataaac agctagcggt tccaaccttg
     2161 gttcccctct ccctttcccc cccctaacgt tactggccga agccgcttgg aataaggccg
     2221 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     2281 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     2341 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctgggag cttcttgaag
     2401 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2461 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2521 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     2581 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     2641 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     2701 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cgggatcctc
     2761 tagagtcgac atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     2821 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     2881 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     2941 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     3001 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     3061 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     3121 ctttgtggca gaggagcagg actgagcggc cgcaatcaac ctctggatta caaaatttgt
     3181 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3241 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3301 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3361 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3421 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3481 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3541 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     3601 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3661 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3721 tccctttggg ccgcctcccc gcagatcccc cgacctcgac ctctggctaa taaaggaaat
     3781 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3841 ggagggcaaa tcatttggtc gagatccctc ggagatctaa gcttcatgtg agcaaaaggc
     3901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4381 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4681 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4741 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4801 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgtgaccc acgctcaccg
     4861 gctccagatt tatcagcaat aaaccagcca gccggaagcg ccgagcgcag aagtggtcct
     4921 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4981 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5041 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5101 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5161 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5221 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5281 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5341 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5401 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5461 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5521 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5581 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5641 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaagat
     5701 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
     5761 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
     5821 tggctcatgt ccaatatgac cgccat
//
