LOCUS       TGEX-LC-mK-Zeo          5159 bp    DNA     circular
DEFINITION  Murine (Mus musculus) Kappa Chain TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,2467..3055,3071..3169)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,4919..4947)
            FT /number="1"
            FT color
            FT 2084..2458
            FT /number="20"
            FT color
            FT join(1041..1099,1132..1454)
            FT /number="14"
            FT translated
            FT 2084..2458
            FT /frame="2"
            FT color
            FT 3243..3862
            FT /number="2"
            FT color
            FT join(1477..1487,1490..1989,1991,1994..2050)
            FT /number="7"
            FT color
            FT join(4017..4157,4159..4877)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..1451
                     /codon_start=1
                     /translation="RADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKID
                     GSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSF
                     NRNEC*"
                     /label=mK*01
     misc_feature    1449..1454
                     /gene
                     /label=Stop
                     /note
     misc_binding    1455..1460
                     /db_xref="REBASE:NheI"
     misc_feature    1477..2050
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(1523..1542)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    1987..1993
                     /label=A7
                     /note
     misc_binding    2072..2077
                     /db_xref="REBASE:XbaI"
     misc_binding    2078..2083
                     /db_xref="REBASE:SalI"
     CDS             2084..2458
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    2459..2466
                     /db_xref="REBASE:NotI"
     misc_feature    2467..3055
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3071..3169
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3186..3191
                     /db_xref="REBASE:BglII"
     misc_binding    3192..3197
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3243..3862)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(3699)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4017..4877)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(4919..4947)
                     /label=AmpR_Promoter
     misc_binding    5010..5015
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcacgg gctgatgctg
     1141 caccaactgt atccatcttc ccaccatcca gtgagcagtt aacatctgga ggtgcctcag
     1201 tcgtgtgctt cttgaacaac ttctacccca aagacatcaa tgtcaagtgg aagattgatg
     1261 gcagtgaacg acaaaatggc gtcctgaaca gttggactga tcaggacagc aaagacagca
     1321 cctacagcat gagcagcacc ctcacgttga ccaaggacga gtatgaacga cataacagct
     1381 atacctgtga ggccactcac aagacatcaa cttcacccat tgtcaagagc ttcaacagga
     1441 atgagtgtta aacagctagc ggttccaacc ttggttcccc tctccctttc ccccccctaa
     1501 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     1561 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     1621 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     1681 gaaggaagca gttcctctgg gagcttcttg aagacaaaca acgtctgtag cgaccctttg
     1741 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     1801 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     1861 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     1921 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     1981 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2041 acgatgataa gcttgccaca acccgggatc ctctagagtc gacatggcca agttgaccag
     2101 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     2161 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     2221 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     2281 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     2341 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     2401 cgccctgcgc gacccggccg gcaactgcgt gcactttgtg gcagaggagc aggactgagc
     2461 ggccgcaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2521 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2581 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2641 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2701 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2761 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2821 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     2881 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2941 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3001 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcagatc
     3061 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3121 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     3181 ctcggagatc taagcttcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4141 gctgcaatga taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag
     4201 ccagccggaa gcgccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4981 cgcacatttc cccgaaaagt gccacctgaa gatctataca ttgaatcaat attggcaatt
     5041 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     5101 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccat
//
