LOCUS       TGEX-SCBlue-Zeo         5917 bp    DNA     circular
DEFINITION  TGEX SCblue Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,3225..3813,3829..3927)
            FT /number="17"
            FT color
            FT join(1207..1227,1242..1247,1266..1271,5677..5705)
            FT /number="1"
            FT color
            FT 2842..3216
            FT /number="20"
            FT color
            FT join(1041..1100,1496..2212)
            FT /number="14"
            FT translated
            FT 1511
            FT /frame="3"
            FT color
            FT join(1186..1206,1278..1298)
            FT /number="19"
            FT color
            FT join(1104..1185,1228..1241,1248..1265,1272..1277,1299..1315)
            FT /number="27"
            FT color
            FT 1101..1103
            FT /number="30"
            FT translated
            FT 2842..3216
            FT /frame="1"
            FT color
            FT 4001..4620
            FT /number="2"
            FT color
            FT join(2235..2245,2248..2747,2749,2752..2808)
            FT /number="7"
            FT color
            FT join(4775..4915,4917..5635)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    1086..1096
                     /db_xref="REBASE:BglI"
     misc_binding    1186..1206
                     /label=operator_O3
     misc_binding    1207..1227
                     /label=CAP_binding
     -35_signal      1242..1247
     -10_signal      1266..1271
     misc_binding    1278..1298
                     /label=operator_O1
     CDS             1316..1486
                     /codon_start=1
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLN*"
                     /gene
                     /label=Alpha-B-gal
                     /note="alpha-fragment of beta-galactosidase"
     CDS             1496..1510
                     /codon_start=1
                     /translation="GPGGP"
                     /gene
                     /label=Linker
                     /note
     misc_binding    1496..1508
                     /db_xref="REBASE:SfiI"
     misc_binding    1497..1507
                     /db_xref="REBASE:BglI"
     CDS             1511..2210
                     /codon_start=1
                     /translation="EPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEV
                     TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS
                     DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH
                     NHYTQKSLSLSPGK*"
                     /gene
                     /label=huIgG1-FC
                     /note
     misc_difference 1524
                     /note="G>C mutation to convert Cys into Ser."
     misc_feature    2207..2212
                     /gene
                     /label=Stop
                     /note
     misc_binding    2213..2218
                     /db_xref="REBASE:NheI"
     misc_feature    2235..2808
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2281..2300)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2745..2751
                     /label=A7
                     /note
     misc_binding    2830..2835
                     /db_xref="REBASE:XbaI"
     misc_binding    2836..2841
                     /db_xref="REBASE:SalI"
     CDS             2842..3216
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3217..3224
                     /db_xref="REBASE:NotI"
     misc_feature    3225..3813
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3829..3927
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3944..3949
                     /db_xref="REBASE:BglII"
     misc_binding    3950..3955
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(4001..4620)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4457)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4775..5635)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5677..5705)
                     /label=AmpR_Promoter
     misc_binding    5768..5773
                     /db_xref="REBASE:BglII"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1141 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     1201 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     1261 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1321 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1381 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1441 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aattaaaata gatagggccc
     1501 gggaggcccc gagcccaaat cttctgacaa aactcacaca tgcccaccgt gcccagcacc
     1561 tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat
     1621 gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga
     1681 ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg
     1741 ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga
     1801 ctggctgaat ggcaaggagt acaagtgcaa ggtgtccaac aaagccctcc cagcccccat
     1861 cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc
     1921 cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt
     1981 ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa
     2041 gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt
     2101 ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct
     2161 gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaataaa cagctagcgg
     2221 ttccaacctt ggttcccctc tccctttccc ccccctaacg ttactggccg aagccgcttg
     2281 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     2341 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     2401 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggga
     2461 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     2521 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     2581 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     2641 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     2701 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     2761 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     2821 ccgggatcct ctagagtcga catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     2881 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     2941 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     3001 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     3061 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     3121 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     3181 aactgcgtgc actttgtggc agaggagcag gactgagcgg ccgcaatcaa cctctggatt
     3241 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3301 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3361 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3421 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3481 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3541 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3601 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3661 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3721 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3781 cgagtcggat ctccctttgg gccgcctccc cgcagatccc ccgacctcga cctctggcta
     3841 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     3901 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatcta agcttcatgt
     3961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4681 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4741 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4801 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4861 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc
     4921 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagc gccgagcgca
     4981 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5041 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5101 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5161 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5221 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5281 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5341 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5401 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5461 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5521 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5581 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5641 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5701 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5761 cacctgaaga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     5821 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     5881 tacatttata ttggctcatg tccaatatga ccgccat
//
