LOCUS       fADL-1e                 7993 bp    DNA     circular
DEFINITION  Filamentous phage cloning vector fADL-1e, complete sequence.
ACCESSION   
VERSION     .2
KEYWORDS    .
COMMENT     Filamentous phage cloning vector constructed by splicing the
            kanamycin resistance determinant of Tn903 into the minus-strand
            origin of bacteriphage fd.  Transduce kanamycin resistance into
            infected host cell.  Double-stranded RF copy number is about 60
            copies in stationary-phase cells.  Yield of physical particles is
            about 5e11 virions/ml.  Make  very small plaques.  Display on
            gene III protein up to 100% with limited polyphage production.
            Foreign inserts can also be spliced into unique HindIII site, and
            are generally stable.
FEATURES             Location/Qualifiers
     CDS             join(7592..7993,1..831)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein pII"
                     /protein_id="AAF75600.1"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA
                     RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIETRYLKSFGLPLNLFDAIRFASDYNRQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA*"
     CDS             496..831
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein pX"
                     /protein_id="AAF75601.1"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
     CDS             843..1106
                     /codon_start=1
                     /transl_table=11
                     /product="cytosolic single-stranded DNA binding protein pV"
                     /protein_id="AAF75603.1"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1108..1209
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pVII"
                     /protein_id="AAF75604.1"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1206..1304
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pIX"
                     /protein_id="AAF75605.1"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1301..1522
                     /codon_start=1
                     /transl_table=11
                     /product="major coat protein pVIII precursor"
                     /protein_id="AAF75598.1"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY
                     IGYAWAMVVVIVGATIGIKLFKKFTSKAS*"
     sig_peptide     1301..1369
     misc_difference 1403
                     /label=M13
                     /note="A in M13, G in fd"
     primer_bind     1486..1510
                     /label=phi8S3
     misc_binding    1565..1570
                     /db_xref="REBASE:SalI"
     CDS             1586..>1648
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPAM"
                     /label=PelB
     primer_bind     1586..1607
                     /label=phiS2
     misc_binding    1607..1614
                     /db_xref="REBASE:NotI"
     misc_binding    1633..1645
                     /db_xref="REBASE:SfiI"
     misc_binding    1634..1644
                     /db_xref="REBASE:BglI"
     misc_binding    1651..1656
                     /db_xref="REBASE:SpeI"
     misc_binding    1657..1669
                     /db_xref="REBASE:SfiI"
     misc_binding    1658..1668
                     /db_xref="REBASE:BglI"
     misc_binding    1674..1679
                     /db_xref="REBASE:XbaI"
     CDS             1681..2901
                     /codon_start=1
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /label=g3p
     primer_bind     complement(1711..1738)
                     /label=psiR2
     primer_bind     complement(1749..1772)
                     /label=psiR3
     misc_binding    2268..2273
                     /db_xref="REBASE:BamHI"
     CDS             2904..3242
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pVI"
                     /protein_id="AAF75606.1"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK*"
     CDS             3245..4291
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein pI"
                     /protein_id="AAF75599.1"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLVTGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSITYIDLCTV
                     SIKKGNSNEIVKCN*"
     CDS             4269..5549
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein IV"
                     /protein_id="AAF75602.1"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQTGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNSQDYI
                     DELPSSDIQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSVDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTVERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*"
     misc_difference 5201..5202
                     /note="Sequencing error in fdtet; AA is also found on f88.4
                     and is reported by G. Smith (e.g. HM453030)."
     misc_feature    5548..5625
                     /note="morphogenetic protein signal sequence; promotes
                     packaging of circular single plus strands into progeny
                     virions; other site"
                     /citation=[3]
     rep_origin      join(5672..5693,7231..7342)
                     /note="minus-strand origin, disrupted by Tn10-derived
                     insert; functionally inactive; minus strand initiated
                     randomly by endogenous bacterial Okazaki fragment priming
                     apparatus"
                     /citation=[3]
                     /citation=[4]
     CDS             complement(6285..7100)
                     /gene="aph(3')-Ia"
                     /note="Confer resistance to kanamycin"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_binding    7066..7071
                     /db_xref="REBASE:XhoI"
     rep_origin      7351..7490
                     /note="plus strand origin"
                     /citation=[3]
     misc_feature    7366^7367
                     /note="pII replication protein nicks plus strand to
                     initiate rolling circle replication. Often one T is missing
                     at that position."
     primer_bind     2665..2687
                     /gene
                     /label=g3_1317_s
                     /note
     CDS             1657..1680
                     /codon_start=1
                     /translation="GPGGLSLE"
                     /gene
                     /label=Linker
                     /note
ORIGIN
        1 aacgctacta ccattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaataaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgaactacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactgtctaa tcctgacctg
      301 ttggaatttg cttccggtct ggttcgcttt gaggctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaattcgct ttgcttctga ctataataga
      421 cagggtaaag acctgatttt tgatttatgg tcattctctt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta caattacccc ctctggcaaa acttcctttg caaaagcctc tcgctatttt
      601 ggtttctatc gtcgtctggt taatgagggt tatgatagtg ttgctcttac catgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgagtgtg gtattcctaa atctcaattg
      721 atgaatcttt ccacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcctcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 aaatgattaa agttgaaatt aaaccatctc aagcgcaatt tactacccgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt gcttgtcaag attactctcg acgaaggtca gccagcgtat gcgcctggtc
     1021 tgtacaccgt gcatctgtcc tcgttcaaag ttggtcagtt cggttctctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     1321 caaagcctcc gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 ttttgtcgac taacgagggc aaatcatgaa atacctattg cctacggcgg ccgctggatt
     1621 gttattactc gcggcccagc cggccatgta actagtggcc cgggaggcct gtctctagaa
     1681 gccgaaactg ttgaaagttg tttagcaaaa cctcatacag aaaattcatt tactaacgtc
     1741 tggaaagacg acaaaacttt agatcgttac gctaactatg agggctgtct gtggaatgct
     1801 acaggcgttg tggtttgtac tggtgacgaa actcagtgtt acggtacatg ggttcctatt
     1861 gggcttgcta tccctgaaaa tgagggtggt ggctctgagg gtggcggttc tgagggtggc
     1921 ggttctgagg gtggcggtac taaacctcct gagtacggtg atacacctat tccgggctat
     1981 acttatatca accctctcga cggcacttat ccgcctggta ctgagcaaaa ccccgctaat
     2041 cctaatcctt ctcttgagga gtctcagcct cttaatactt tcatgtttca gaataatagg
     2101 ttccgaaata ggcagggtgc attaactgtt tatacgggca ctgttactca aggcactgac
     2161 cccgttaaaa cttattacca gtacactcct gtatcatcaa aagccatgta tgacgcttac
     2221 tggaacggta aattcagaga ctgcgctttc cattctggct ttaatgagga tccattcgtt
     2281 tgtgaatatc aaggccaatc gtctgacctg cctcaacctc ctgtcaatgc tggcggcggc
     2341 tctggtggtg gttctggtgg cggctctgag ggtggcggct ctgagggtgg cggttctgag
     2401 ggtggcggct ctgagggtgg cggttccggt ggcggctccg gttccggtga ttttgattat
     2461 gaaaaaatgg caaacgctaa taagggggct atgaccgaaa atgccgatga aaacgcgcta
     2521 cagtctgacg ctaaaggcaa acttgattct gtcgctactg attacggtgc tgctatcgat
     2581 ggtttcattg gtgacgtttc cggccttgct aatggtaatg gtgctactgg tgattttgct
     2641 ggctctaatt cccaaatggc tcaagtcggt gacggtgata attcaccttt aatgaataat
     2701 ttccgtcaat atttaccttc tttgcctcag tcggttgaat gtcgccctta tgtctttggc
     2761 gctggtaaac catatgaatt ttctattgat tgtgacaaaa taaacttatt ccgtggtgtc
     2821 tttgcgtttc ttttatatgt tgccaccttt atgtatgtat tttcgacgtt tgctaacata
     2881 ctgcgtaata aggagtctta atcatgccag ttcttttggg tattccgtta ttattgcgtt
     2941 tcctcggttt ccttctggta actttgttcg gctatctgct tactttcctt aaaaagggct
     3001 tcggtaagat agctattgct atttcattgt ttcttgctct tattattggg cttaactcaa
     3061 ttcttgtggg ttatctctct gatattagcg cacaattacc ctctgatttt gttcagggcg
     3121 ttcagttaat tctcccgtct aatgcgcttc cctgttttta tgttattctc tctgtaaagg
     3181 ctgctatttt catttttgac gttaaacaaa aaatcgtttc ttatttggat tgggataaat
     3241 aaatatggct gtttattttg taactggcaa attaggctct ggaaagacgc tcgttagcgt
     3301 tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc ttgatttaag
     3361 gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc ttagaatacc
     3421 ggataagcct tctatttctg atttgcttgc tattggtcgt ggtaatgatt cctacgacga
     3481 aaataaaaac ggtttgcttg ttcttgatga atgcggtact tggtttaata cccgttcatg
     3541 gaatgacaag gaaagacagc cgattattga ttggtttctt catgctcgta aattgggatg
     3601 ggatattatt tttcttgttc aggatttatc tattgttgat aaacaggcgc gttctgcatt
     3661 agctgaacac gttgtttatt gtcgccgtct ggacagaatt actttaccct ttgtcggcac
     3721 tttatattct cttgttactg gctcaaaaat gcctctgcct aaattacatg ttggtgttgt
     3781 taaatatggt gattctcaat taagccctac tgttgagcgt tggctttata ctggtaagaa
     3841 tttatataac gcatatgaca ctaaacaggc tttttccagt aattatgatt caggtgttta
     3901 ttcatattta accccttatt tatcacacgg tcggtatttc aaaccattaa atttaggtca
     3961 gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt gtcttgcgat
     4021 aggatttgca tcagcattta catatagtta tataacccaa cctaagccgg aggttaaaaa
     4081 ggtagtctct cagacctatg attttgataa attcactatt gactcttctc agcgtcttaa
     4141 tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata gcgacgattt
     4201 acagaagcaa ggttattcca tcacatatat tgatttatgt actgtttcaa ttaaaaaagg
     4261 taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt gtttcatcat
     4321 cttcttttgc tcaagtaatt gaaatgaata attcgcctct gcgcgatttc gtgacttggt
     4381 attcaaagca aacaggtgaa tctgttattg tctcacctga tgttaaaggt acagtgactg
     4441 tatattcctc tgacgttaag cctgaaaatt tacgcaattt ctttatctct gttttacgtg
     4501 ctaataattt tgatatggtt ggctctaatc cttccataat tcagaaatat aacccaaata
     4561 gtcaggatta tattgatgaa ttgccatcat ctgatattca ggaatatgat gataattccg
     4621 ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaaca tttaaaatta
     4681 ataacgttcg cgcaaaggat ttaataaggg ttgtagaatt gtttgttaaa tctaatacat
     4741 ctaaatcctc aaatgtatta tctgttgatg gttctaactt attagtagtt agcgccccta
     4801 aagatatttt agataacctt ccgcaatttc tttctactgt tgatttgcca actgaccaga
     4861 tattgattga aggattaatt ttcgaggttc agcaaggtga tgctttagat ttttcctttg
     4921 ctgctggctc tcagcgcggc actgttgctg gtggtgttaa tactgaccgt ctaacctctg
     4981 ttttatcttc tgcgggtggt tcgttcggta tttttaacgg cgatgtttta gggctatcag
     5041 ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgcctcgt attcttacgc
     5101 tttcaggtca gaagggttct atttctgttg gccagaatgt cccttttatt actggtcgtg
     5161 taactggtga atctgccaat gtaaataatc catttcagac aattgagcgt caaaatgttg
     5221 gtatttctat gagtgttttt cccgttgcaa tggctggcgg taatattgtt ttagatataa
     5281 ccagtaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt actaatcaaa
     5341 gaagtattgc gacaacggtt aatttgcgtg atggtcagac tcttttgctc ggtggcctca
     5401 ctgattacaa aaacacttct caagattctg gtgtgccgtt cctgtctaaa atccctttaa
     5461 tcggcctcct gtttagctcc cgttctgatt ctaacgagga aagcacgttg tacgtgctcg
     5521 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5581 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5641 ccttcctttc tcgccacgtt ctccggcttt ccccgtcaag ctctaaatcg ggggatctcg
     5701 ggaaaagcgt tggtgaccaa aggtgccttt tatcatcact ttaaaaataa aaaacaatta
     5761 ctcagtgcct gttataagca gcaattaatt atgattgatg cctacatcac aacaaaaact
     5821 gatttaacaa atggttggtc tgccttagaa agtatatttg aacattatct tgattatatt
     5881 attgataata ataaaaacct tatccctatc caagaagtga tgcctatcat tggttggaat
     5941 gaacttgaaa aaattagcct tgaatacatt actggtaagg taaacgccat tgtcagcaaa
     6001 ttgatccaag agaaccaact taaagcttat gatgatgatg tgcttaaaaa cttactcaat
     6061 ggctggttta tgcatatcgc aatacatgcg aaaaacctaa aagagcttgc cgataaaaaa
     6121 ggccaattta ttgctattta ccgcggcttt ttattgagct tgaaagataa ataaaataga
     6181 taggttttat ttgaagctaa atcttcttta tcgtaaaaaa tgccctcttg ggttatcaag
     6241 agggtcatta tatttcgcgg aataaaccaa ttaaccaatt ctgattagaa aaactcatcg
     6301 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     6361 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     6421 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     6481 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     6541 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     6601 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     6661 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     6721 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     6781 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     6841 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     6901 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     6961 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     7021 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt
     7081 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     7141 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     7201 acgtggcttt tgttgacaaa gggaatcata gatcccttta gggttccgat ttagtgcttt
     7261 acggcacctc gacctccaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     7321 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7381 gttccaaact ggaacaacac tcacaactaa cccggcctat tcttttgatt tataaggatt
     7441 tttgtcattt tctgcttact ggttaaaaaa taagctgatt taacaaatat ttaacgcgaa
     7501 atttaacaaa acattaacgt ttacaattta aatatttgct tatacaatca tcctgttttt
     7561 ggggcttttc tgattatcaa tcggggtaca tatgattgac atgctagttt tacgattacc
     7621 gttcatcgat tctcttgttt gctccagact ttcaggtaat gacctgatag cctttgtaga
     7681 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
     7741 tattgacggt gatttgactg tctccggcct ttctcacccg tttgaatctt tgcctactca
     7801 ttactccggc attgcattta aaatatatga gggttctaaa aatttttatc cctgcgttga
     7861 aattaaggct tcaccagcaa aagtattaca gggtcataat gtttttggta caaccgattt
     7921 agctttatgc tctgaggctt tattgcttaa ttttgctaac tctctgcctt gcttgtacga
     7981 tttattggat gtt
//

