LOCUS       pADL-100                3943 bp    DNA     circular
DEFINITION  Phage display vector pADL-100, complete sequence.
ACCESSION   
VERSION     .1
KEYWORDS    .
COMMENT     This file is best viewed using GenBeans DNA sequence analysis
            software (www.genbeans.org).
FEATURES             Location/Qualifiers
     promoter        56..84
                     /label=AmpR_Promoter
                     /color="9"
     CDS             126..986
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     rep_origin      1141..1760
                     /label=pMB1
                     /gene="pBR322 origin"
     primer_bind     1770..1790
                     /label=pmb630_s
     misc_binding    1890..1910
                     /label=O3
                     /color="18"
     misc_signal     1911..1931
                     /label=CAP_binding_site
                     /note="CAP binding site"
     -35_signal      1946..1951
     -10_signal      1970..1975
     misc_feature    1981
                     /label=start
                     /note="transcription start of the lac operon"
     misc_binding    1982..2002
                     /label=O1
                     /color="18"
     primer_bind     1990..2019
                     /label=phiS4
     misc_binding    2004..2009
                     /db_xref="REBASE:EcoRI"
     CDS             2025..2087
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     primer_bind     2025..2046
                     /label=phiS2
     misc_binding    2046..2053
                     /db_xref="REBASE:NotI"
     misc_binding    2072..2084
                     /db_xref="REBASE:SfiI"
     misc_binding    2073..2083
                     /db_xref="REBASE:BglI"
     misc_binding    2087..2092
                     /db_xref="REBASE:SpeI"
     CDS             2093..2149
                     /codon_start=1
                     /translation="GPGGQGGGSEGGGSLEGAP"
                     /gene
                     /label=Linker
                     /note
     misc_binding    2093..2105
                     /db_xref="REBASE:SfiI"
     misc_binding    2094..2104
                     /db_xref="REBASE:BglI"
     misc_binding    2134..2141
                     /db_xref="REBASE:AbsI"
     misc_binding    2135..2140
                     /db_xref="REBASE:XhoI"
     misc_binding    2141..2148
                     /db_xref="REBASE:AscI"
     misc_binding    2142..2147
                     /db_xref="REBASE:BssHII"
     CDS             2150..3370
                     /gene="gene III"
                     /codon_start=1
                     /product="pIII"
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /label=g3p
     primer_bind     complement(2180..2207)
                     /label=psiR2
     primer_bind     complement(2218..2241)
                     /label=psiR3
     misc_binding    2737..2742
                     /db_xref="REBASE:BamHI"
     misc_binding    3371..3376
                     /db_xref="REBASE:NheI"
     terminator      3380..3493
                     /label=lt1_terminator
     rep_origin      3614..3920
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggcctaacta gtggcccggg
     2101 aggccaaggc ggtggttctg agggtggtgg ctccctcgag ggcgcgccag ccgaaactgt
     2161 tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga
     2221 caaaacttta gatcgttacg ctaactatga gggctgtctg tggaatgcta caggcgttgt
     2281 ggtttgtact ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat
     2341 ccctgaaaat gagggtggtg gctctgaggg tggcggttct gagggtggcg gttctgaggg
     2401 tggcggtact aaacctcctg agtacggtga tacacctatt ccgggctata cttatatcaa
     2461 ccctctcgac ggcacttatc cgcctggtac tgagcaaaac cccgctaatc ctaatccttc
     2521 tcttgaggag tctcagcctc ttaatacttt catgtttcag aataataggt tccgaaatag
     2581 gcagggtgca ttaactgttt atacgggcac tgttactcaa ggcactgacc ccgttaaaac
     2641 ttattaccag tacactcctg tatcatcaaa agccatgtat gacgcttact ggaacggtaa
     2701 attcagagac tgcgctttcc attctggctt taatgaggat ccattcgttt gtgaatatca
     2761 aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct ggcggcggct ctggtggtgg
     2821 ttctggtggc ggctctgagg gtggcggctc tgagggtggc ggttctgagg gtggcggctc
     2881 tgagggtggc ggttccggtg gcggctccgg ttccggtgat tttgattatg aaaaaatggc
     2941 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     3001 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     3061 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     3121 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     3181 tttaccttct ttgcctcagt cggttgaatg tcgcccttat gtctttggcg ctggtaaacc
     3241 atatgaattt tctattgatt gtgacaaaat aaacttattc cgtggtgtct ttgcgtttct
     3301 tttatatgtt gccaccttta tgtatgtatt ttcgacgttt gctaacatac tgcgtaataa
     3361 ggagtcttaa gctagctaac agtcactatg aatcaactac ttagatggta ttagtgacct
     3421 gtaacagagc attagcgcaa ggtgattttt gtcttcttgc gctaattttt tgtcatcaaa
     3481 cctgtcgcac tccttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3541 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3601 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3661 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3721 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3781 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3841 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3901 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtg
//
