LOCUS       pADL-20c                3930 bp    DNA     circular
DEFINITION  Phage display vector pADL-20c, complete sequence.
ACCESSION   
VERSION     .1
KEYWORDS    .
COMMENT     Phage display vector pADL-20c, complete sequence.
FEATURES             Location/Qualifiers
     promoter        56..84
                     /label=P3 Promoter
     CDS             126..986
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     rep_origin      1141..1760
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_binding    1890..1910
                     /label=O3_operator
     misc_signal     1911..1931
                     /note="CAP binding site"
                     /label=CAP_binding_site
     -35_signal      1946..1951
     -10_signal      1970..1975
     misc_feature    1981
                     /label=start
                     /note="transcription start of the lac operon"
     misc_binding    1982..2002
                     /label=O1_operator
     primer_bind     1990..2019
                     /label=phiS4
     misc_binding    2004..2009
                     /db_xref="REBASE:EcoRI"
     CDS             2025..2087
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     primer_bind     2025..2046
                     /label=phiS2
     misc_binding    2046..2053
                     /db_xref="REBASE:NotI"
     misc_binding    2072..2084
                     /db_xref="REBASE:SfiI"
     misc_binding    2073..2083
                     /db_xref="REBASE:BglI"
     misc_binding    2087..2092
                     /db_xref="REBASE:SpeI"
     misc_binding    2093..2105
                     /db_xref="REBASE:SfiI"
     misc_binding    2094..2104
                     /db_xref="REBASE:BglI"
     CDS             2138..3358
                     /gene="gene III"
                     /codon_start=1
                     /product="pIII"
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /label=g3p
     primer_bind     complement(2168..2195)
                     /label=psiR2
     primer_bind     complement(2206..2229)
                     /label=psiR3
     misc_binding    2725..2730
                     /db_xref="REBASE:BamHI"
     misc_binding    3359..3364
                     /db_xref="REBASE:NheI"
     terminator      3368..3480
                     /note="lambda t1 terminator"
     rep_origin      3601..3907
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
     misc_feature    2108..2125
                     /gene
                     /label=His-Tag
                     /note
     CDS             2093..2138
                     /codon_start=1
                     /translation="GPGGQHHHHHHGAS*"
                     /gene
                     /label=Linker
                     /note
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggcctaacta gtggcccggg
     2101 aggccaacac catcaccacc atcatggcgc atcttaggcc gaaactgttg aaagttgttt
     2161 agcaaaacct catacagaaa attcatttac taacgtctgg aaagacgaca aaactttaga
     2221 tcgttacgct aactatgagg gctgtctgtg gaatgctaca ggcgttgtgg tttgtactgg
     2281 tgacgaaact cagtgttacg gtacatgggt tcctattggg cttgctatcc ctgaaaatga
     2341 gggtggtggc tctgagggtg gcggttctga gggtggcggt tctgagggtg gcggtactaa
     2401 acctcctgag tacggtgata cacctattcc gggctatact tatatcaacc ctctcgacgg
     2461 cacttatccg cctggtactg agcaaaaccc cgctaatcct aatccttctc ttgaggagtc
     2521 tcagcctctt aatactttca tgtttcagaa taataggttc cgaaataggc agggtgcatt
     2581 aactgtttat acgggcactg ttactcaagg cactgacccc gttaaaactt attaccagta
     2641 cactcctgta tcatcaaaag ccatgtatga cgcttactgg aacggtaaat tcagagactg
     2701 cgctttccat tctggcttta atgaggatcc attcgtttgt gaatatcaag gccaatcgtc
     2761 tgacctgcct caacctcctg tcaatgctgg cggcggctct ggtggtggtt ctggtggcgg
     2821 ctctgagggt ggcggctctg agggtggcgg ttctgagggt ggcggctctg agggtggcgg
     2881 ttccggtggc ggctccggtt ccggtgattt tgattatgaa aaaatggcaa acgctaataa
     2941 gggggctatg accgaaaatg ccgatgaaaa cgcgctacag tctgacgcta aaggcaaact
     3001 tgattctgtc gctactgatt acggtgctgc tatcgatggt ttcattggtg acgtttccgg
     3061 ccttgctaat ggtaatggtg ctactggtga ttttgctggc tctaattccc aaatggctca
     3121 agtcggtgac ggtgataatt cacctttaat gaataatttc cgtcaatatt taccttcttt
     3181 gcctcagtcg gttgaatgtc gcccttatgt ctttggcgct ggtaaaccat atgaattttc
     3241 tattgattgt gacaaaataa acttattccg tggtgtcttt gcgtttcttt tatatgttgc
     3301 cacctttatg tatgtatttt cgacgtttgc taacatactg cgtaataagg agtcttaagc
     3361 tagctaacag tcctatgaat caactactta gatggtatta gtgacctgta acagagcatt
     3421 agcgcaaggt gatttttgtc ttcttgcgct aattttttgt catcaaacct gtcgcactcc
     3481 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3541 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3601 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3661 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3721 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3781 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3841 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3901 ttaatgcgcc gctacagggc gcgtcaggtg
//
