LOCUS       pADL-22c                3957 bp    DNA     circular
DEFINITION  Phage display vector pADL-22c, complete sequence.
ACCESSION   
VERSION     .1
KEYWORDS    .
COMMENT     Phage display vector pADL-22c, complete sequence.
FEATURES             Location/Qualifiers
     promoter        56..84
                     /label=P3 Promoter
     CDS             126..986
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     rep_origin      1141..1760
                     /label=pMB1
                     /gene="pBR322 origin"
     primer_bind     1770..1790
                     /label=pmb630s
     misc_binding    1890..1910
                     /label=O3_operator
     misc_signal     1911..1931
                     /note="CAP binding site"
                     /label=CAP_binding_site
     -35_signal      1946..1951
     -10_signal      1970..1975
     misc_feature    1981
                     /label=start
                     /note="transcription start of the lac operon"
     misc_binding    1982..2002
                     /label=O1_operator
     primer_bind     1990..2019
                     /label=phiS4
     misc_binding    2004..2009
                     /db_xref="REBASE:EcoRI"
     CDS             2025..2087
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     primer_bind     2025..2046
                     /label=phiS2
     misc_binding    2046..2053
                     /db_xref="REBASE:NotI"
     misc_binding    2072..2084
                     /db_xref="REBASE:SfiI"
     misc_binding    2073..2083
                     /db_xref="REBASE:BglI"
     misc_binding    2087..2092
                     /db_xref="REBASE:SpeI"
     misc_binding    2093..2105
                     /db_xref="REBASE:SfiI"
     misc_binding    2094..2104
                     /db_xref="REBASE:BglI"
     CDS             2165..3385
                     /gene="gene III"
                     /codon_start=1
                     /product="pIII"
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
     primer_bind     complement(2195..2222)
                     /label=psiR2
     primer_bind     complement(2233..2256)
                     /label=psiR3
     misc_binding    2752..2757
                     /db_xref="REBASE:BamHI"
     misc_binding    3386..3391
                     /db_xref="REBASE:NheI"
     terminator      3395..3507
                     /note="lambda t1 terminator"
     rep_origin      3628..3934
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
     CDS             2093..2164
                     /codon_start=1
                     /translation="GPGGQHHHHHHGAYPYDVPDYAS*"
                     /gene
                     /label=Linker
                     /note
     misc_feature    2108..2125
                     /gene
                     /label=His-Tag
                     /note
     misc_feature    2132..2158
                     /gene
                     /label=HA-Tag
                     /note
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggcctaacta gtggcccggg
     2101 aggccaacac catcaccacc atcatggcgc atatccgtat gatgtgccgg actatgcttc
     2161 ttaggccgaa actgttgaaa gttgtttagc aaaacctcat acagaaaatt catttactaa
     2221 cgtctggaaa gacgacaaaa ctttagatcg ttacgctaac tatgagggct gtctgtggaa
     2281 tgctacaggc gttgtggttt gtactggtga cgaaactcag tgttacggta catgggttcc
     2341 tattgggctt gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg
     2401 tggcggttct gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg
     2461 ctatacttat atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc
     2521 taatcctaat ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa
     2581 taggttccga aataggcagg gtgcattaac tgtttatacg ggcactgtta ctcaaggcac
     2641 tgaccccgtt aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc
     2701 ttactggaac ggtaaattca gagactgcgc tttccattct ggctttaatg aggatccatt
     2761 cgtttgtgaa tatcaaggcc aatcgtctga cctgcctcaa cctcctgtca atgctggcgg
     2821 cggctctggt ggtggttctg gtggcggctc tgagggtggc ggctctgagg gtggcggttc
     2881 tgagggtggc ggctctgagg gtggcggttc cggtggcggc tccggttccg gtgattttga
     2941 ttatgaaaaa atggcaaacg ctaataaggg ggctatgacc gaaaatgccg atgaaaacgc
     3001 gctacagtct gacgctaaag gcaaacttga ttctgtcgct actgattacg gtgctgctat
     3061 cgatggtttc attggtgacg tttccggcct tgctaatggt aatggtgcta ctggtgattt
     3121 tgctggctct aattcccaaa tggctcaagt cggtgacggt gataattcac ctttaatgaa
     3181 taatttccgt caatatttac cttctttgcc tcagtcggtt gaatgtcgcc cttatgtctt
     3241 tggcgctggt aaaccatatg aattttctat tgattgtgac aaaataaact tattccgtgg
     3301 tgtctttgcg tttcttttat atgttgccac ctttatgtat gtattttcga cgtttgctaa
     3361 catactgcgt aataaggagt cttaagctag ctaacagtcc tatgaatcaa ctacttagat
     3421 ggtattagtg acctgtaaca gagcattagc gcaaggtgat ttttgtcttc ttgcgctaat
     3481 tttttgtcat caaacctgtc gcactcctta atattttgtt aaaattcgcg ttaaattttt
     3541 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3601 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3661 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3721 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3781 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3841 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3901 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtg
//
