EX-PRESS 293™

EX-PRESS 293™

Technical Description

EX-PRESS 293™ is a HEK293 cell line variant adapted for serum-free suspension culture in chemically defined media optimized for high-density growth, efficient transfection, and robust recombinant protein production. Its specialized protocol minimizes transfection requirements while maintaining protein yield and quality. For recombinant antibody production, we recommend the TGEX™ mammalian expression vector series.

Features of EX-PRESS 293™ cells include:

• Human-derived cell line adapted high density suspension culture and chemically-defined, serum-free media.

• Optimized for 6-day fed-batch recombinant protein expression.

• Designed to work seamlessly with TGEX™ mammalian expression vectors to produce a wide range of antibody types and formats.

• Compatible with commonly used transfection reagents.

• Utilizes a unique transfection protocol to significantly lower cost of transfection.

• Flexibility of generating biotinylated proteins without additional post-purification processing (EX-PRESS™ 293-BL).

Frozen cells are available as a single cryovial, or as a multipack to eliminate the need to expand and bank cells before use. Each cryovial contains 1 ml of 1 x 10⁷ cells/ml in BalanCD HEK293 with 10% DMSO. Cells should be thawed directly in complete BalanCD HEK293 supplemented with 2-mM L-glutamine.

Thawed cells are grown in a humidified CO₂ incubator on a shaking platform in multi-well plates to large shake flasks. Additionally, EX-PRESS 293™ cells take advantage of the unique shape of Thomson Optimum Growth® shake flasks in transfecting cells at high density followed by increasing production volume without needing to change vessels. This allows for increased yields at lower cost and shaker footprint, without impacting cellular growth. Recombinant protein production follows a simple fed-batch protocol with harvest six days post-transfection.

Applications

• Recombinant protein expression.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Product name: EX-PRESS 293™
Expression system: Mammalian
Cell type: Human embryonic kidney
Culture type: Suspension
Media recommendation: BalanCD HEK293 + 2-mM L-glutamine
Culture conditions: Humidified incubator set to 37°C and 8% CO₂ with shaking at 150 rpm (20 mm orbital diameter)

Physical Characteristics:

Concentration: 1 x 10⁷ cells/ml
Product Size: 1 ml
Buffer: BalanCD HEK293 with 10% (v/v) DMSO
Storage Temperature: Shipped on dry ice, store in liquid nitrogen upon receipt

Quality Control & Certification of Analysis

Cells are certified to meet all specifications, including absence of Mycoplasma species by qPCR.

Notes

Thawing EX-PRESS™ 293 cells:

To ensure the highest level of viability, thaw a cryovial and initiate culture upon receipt. If immediate culturing of cells is not required, transfer frozen cryovial(s) to storage in liquid nitrogen. Cryovials stored at -70°C will result in loss of viability.

Transient transfection and protein production:

EX-PRESS 293™ cell lines are compatible with commercially available transient transfection systems and mammalian expression vectors. Protocols for transfection and a short 6-day fed-batch recombinant protein production are available in the product manual.

Related Products

EX-PRESS 293™ cells (Pack 6 x 1 ml)

EX-PRESS 293™-BL cells

EX-PRESS 293™-BL cells (Pack 6 x 1 ml)

TGEX Antibody Expression Vector Series