SS320 Chemically Competent Cells

SS320 Chemically Competent Cells

Technical Description

SS320 Chemically Competent cells are ready-to-transform bacterial SS320 cells ideal for the isolation of single clones containing a phagemid or an M13-based phage vector.

Applications

• Phage display

• Display of non-amber-containing phage-fusion proteins

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Product Information

Phenotype: SS320 hsdR2 mcrA0 araD139 Δ(araA-leu)7697 ΔlacX74 galK16 galE15(GalS) λe14^- rpsL150(Str^R) spoT1 thi F'[proAB⁺ lacI^q lacZΔM15 Tn10 (tet^r)]
Transformation Efficiency: >1E8 cfu/µg pUC18 DNA
Storage temperature: -80°C
Product size: 10x 0.1 ml
Reagents Supplied: pUC18 Control DNA 50 pg/µl
Materials Required but not Supplied: SOC media, 2xYT media, 2xYT Agar plates with antibiotic

Quality Control & Certification of Analysis

Transformation Efficiency:

Titer in cfu/µg is determined by plating appropriate dilutions in 2xYT medium on agar plates supplemented with ampicillin no more than 55 min post-heat shock with pUC18 DNA.

Contaminations:

No growth with common antibiotics
No M13 contamination

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