EBY100 Yeast Cells

EBY100 Yeast Cells

Technical Description

Saccharomyces cerevisiae EBY100 is a specialized MATα yeast strain engineered for high-efficiency surface display and protein screening. Derived from the protease-deficient BJ5465 background, this strain is designed to maintain the structural integrity of displayed proteins, making it an ideal host for directed evolution and antibody library maturation. EBY100 features a genomic integration of the AGA1 anchoring protein under the galactose-inducible GAL1 promoter, ensuring that the display machinery is only activated when desired. The system operates using the natural α-agglutinin yeast adhesion mechanism. While the EBY100 host cell provides the cell-wall-bound Aga1p subunit, the user-supplied vector (e.g., pCTCont2, pYD1 or pYDL series) expresses the target protein fused to the Aga2p subunit. Once induced in galactose-supplemented media, these two subunits link via disulfide bonds, effectively tethering the target protein to the extracellular matrix. With leu2 and trp1 selection markers and a robust growth profile, EBY100 provides a reliable, high-density display platform compatible with both magnetic bead enrichment and FACS-based screening.

Applications

• Yeast display.

• Antibody engineering.

• Affinity maturation.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Composition: EBY100 MATα AGA1::GAL1-AGA1::URA3 ura3-52 trp1 leu2-Δ200 his3-Δ200 pep4::HIS3 prbd1.6R can1 GALin freezing medium.
Titer: 50 OD₆₀₀, >2E8 cfu/mL
Doubling Time: ~90 min at 30°C and 250 rpm in YPD medium
Storage Temperature: -80°C

Product Size:

1x 0.5 mL

Quality Control & Certification of Analysis

Concentration:

Absorption is measured at 600 nm in 1 cm path cuvette after dilution in freezing medium.

Titer:

Titer in cfu/ml is determined by plating appropriate dilutions in YPD medium on agar plates without antibiotics.

Contaminations:

No bacterial colonies are found after plating 50 µl of cells on 2xYT agar plates. No prototrophic yeast contaminants observed after plating cells on tryptophan dropout media.

Certification:

Product meets all specifications.

Notes

Thawing EBY100 cells:

To ensure the highest level of viability, thaw and initiate culture upon receipt. If immediate culturing of cells is not required, transfer to storage at -80°C.

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