TGEX-HC-hG1[YTE-KF]-Zeo

TGEX-HC-hG1[YTE-KF]-Zeo

Heavy Chain Cloning Vector for the Mammalian Expression of Human IgG1 Antibodies with M252Y, S254T, T256E, H433K, and N434F mutations known to increase antibody serum half-life.

Technical Description

The TGEX™ vector series is designed for the rapid expression of antibody molecules by transient gene expression in mammalian cell suspension cultures. TGEX™-HC-hG1[YTE-KF]-Zeo expresses the constant region of the human IgG1 heavy chain with M252Y, S254T, T256E, H433K, and N434F mutations known to increase antibody serum half-life (Vaccaro 2005). Expression of full length antibody molecules is achieved by co-transfection with a light chain variable region cloned into one of the TGEX™-LC vector series. Using transient transfection technologies yields of antibody between 20 mg/L and 200 mg/L and more in serum-free conditions can be obtained in just a few days.

Applications

• Mammalian expression of full length antibodies by transient gene expression.

• Selection of stable transfectants using Zeocin™.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Plasmid Size: 5828
Promoter: CMV + TPL
Leader Peptide: pelBK
Cloning Site: Double BsaI
Fusion Protein: full length IgG1 human constant region with M252Y, S254T, T256E, H433K, and N434F mutations
Selection: ampicillin (bacteria), zeocin™ (mammalian)
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Product Sequence:

The entire plasmid sequence was confirmed by the Sanger method.

Certification:

Product meets all specifications.

References

1. Vaccaro, C., Zhou, J., Ober, R. et al. 2005. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol 23, 1283–1288